Customer Review For FAAH Polyclonal Antibody (STJ23602)
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The document provides a customer review of the FAH polyclonal antibody (stj23602), detailing its effective use in immunofluorescence on dorsal root ganglia. The review, conducted by Natalia Malek from the Institute of Pharmacology, praised the antibody's performance, leading to a purchase decision. Additionally, it promotes a risk-free trial program allowing customers to sample the product before buying.
Customer Review For FAAH Polyclonal Antibody (STJ23602)
- 1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: FAAH Polyclonal Antibody (STJ23602)
- 2. WHAT WAS USED? Primary antibody: Product Catalog No: STJ23602. FAAH antibody. 20µl Provider: St John's Laboratory Dilution ratio: 1:50 Application: Immunofluorescence. Image: Dorsal root ganglia stained with FAAH antibody.
- 3. WHAT WAS THE PROTOCOL? 1. Samples were frozen and stored at -80°C until sections were cut at 12 μm thick and collected onto gelatin-coated slides (Menzel GmbH, Braunschweig, Germany). 2. For immunofluorescence labelling, serial sections of DRG were hydrated in PB buffer (pH=7.4) for 15 mins. 3. Cell membranes were permeabilised in PB containing 0.1% Triton X-100 and afterwards incubated for 1 h in 10% normal goat serum (NGS, Jackson ImmunoResearch Laboratories, West Grove, USA) in PB containing 0.4% Triton X-100 (blocking solution). 4. Sections were incubated for 48 hrs at 4°C in a humid chamber with the respective primary antibodies (FAAH, St. John’s laboratory; 1:50). 5. After three washes in PB, triple immunofluorescence was revealed by incubation at 4°C in for 24 hrs in the dark with the appropriate fluorochrome-conjugated secondary antibody. 6. Sections were washed 3 times for 15 mins with PB and coverslipped with Aquatex mounting medium (Merck, Darmstadt, Germany). 7. The sections processed for immunofluorescence were studied with a fluorescence microscope (DM RXA2) with confocal scanner (TCS SL) (Leica Microsystems GmbH, Manheim, Germany) equipped with the following lasers: Ar 488, He-Ne 543, He-Ne 633.
- 4. WHAT DID THE CUSTOMER THINK? Testing results were provided by Natalia Malek from the Institute of Pharmacology Polish Academy of Sciences. They stated that “We completed evaluation of FAAH antibody. It worked really well, so we decided to purchase a vial from St John’s Laboratory.”
- 5. “WE INVITE YOU TO TEST OUR PRODUCTS” Our aim is to not only connect our customers with the best product, but to also give them the opportunity to test the product’s performance risk free.
- 6. Contact Us: [email protected] 1. Choose 5 Trial Size Samples from our free sample list 2. Complete the free sample application: http://www.stjohnslabs.com/free-sample 3. Receive your antibody 4. Submit your review 5. Receive your 30% off your next order Get Your Free Samples in 5 Easy Steps
- 7. Follow Us for Weekly Independent Validation Reviews @StJohnsLabsSt John's Laboratory Ltd St John's Laboratory Ltd St John's Laboratory Ltd http://www.slideshare.net/stjohnslabs