Antibody Customer Review For HIF-1α Polyclonal Antibody (STJ93498)
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The document reviews the performance of St. John's Laboratory's HIF-1α polyclonal antibody (stj93498) in a western blot analysis using HeLa cells. According to Dr. Manuela Lanzafame, the antibody successfully detected a band close to the predicted molecular weight of ~92 kDa and two additional bands. The document also promotes a risk-free trial for customers interested in testing the product, providing a 30% discount on future orders upon submitting a review.
Antibody Customer Review For HIF-1α Polyclonal Antibody (STJ93498)
- 1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: HIF-1α Polyclonal Antibody (STJ93498)
- 2. WHAT WAS USED? Primary antibody: STJ93498 HIF-1α Polyclonal Antibody Provider: St John's Laboratory Dilution ratio: 1:500 Application: Western Blot. Materials for validation: HeLa cell line
- 3. WHAT WAS THE PROTOCOL? Treatment of materials: When cells were cultured to 90% plating density, RIPA buffer with protease inhibitors (Roche) and equal volume of 2X loading buffer were added for 10 min water bath at 100˚C. Gel electrophoresis: 4-20% polyacrylamide gel (Bio-Rad), constant voltage 180V, 40 min. Transfer: 0.25 nm nitrocellulose membrane, by using the Trans-Blot Turbo transfer system (Bio-Rad) for 7 minutes. Blocking: 1X TBST with 5% skimmed milk powder for 1 hr at room temperature. Primary antibody probing: Primary antibody was added to the solution of 1X TBST with 5% skimmed milk powder, 4˚C overnight. Membrane wash: 1X TBST wash for 3 times. Secondary antibody probing: Secondary antibody was added to the solution of 1X TBST with 5% skimmed milk powder with 1:10000 dilution ratio, for 1 hr. Membrane wash: 1X TBST wash for 3 times. Visualization: Chemiluminescent signal obtained by using the SuperSignal West Chemiluminescent Substrate (Pierce) were detected using the ChemiDoc™ XRS system (BioRad) with an exposure time of 1 minutes.
- 4. WHAT WAS THE PROTOCOL? Gel electrophoresis information：4-20% Mini-Protean TGX Gels (Bio-Rad), constant voltage 180 V, 40 min. Transfer information：0.25 nm nitrocellulose membrane, by using the Trans-Blot Turbo transfer system (Bio-Rad) for 7 minutes. No. Antigen Loading amount Primary antibody Primary antibody dilution ratio Secondary antibody dilution ratio Target band (KDa) Visualization time 1 HeLa lysate 20 µg STJ93498. HIF-1α Polyclonal Antibody 1:500 1:10000 ~92KDa 1 min
- 5. WHAT DID THE CUSTOMER THINK? Testing results were provided by Manuela Lanzafame, PhD from Istituto di Genetica Molecolare-CNR, Italy. They stated that “In HeLa cells the STJ93498 HIF-1α antibody detects a band of the predicted molecular weight of ~92 KDa and two additional bands.”
- 6. “WE INVITE YOU TO TEST OUR PRODUCTS” Our aim is to not only connect our customers with the best product, but to also give them the opportunity to test the product’s performance risk free.
- 7. Contact Us: [email protected] 1. Choose 5 Trial Size Samples from our free sample list 2. Complete the free sample application: http://www.stjohnslabs.com/free-sample 3. Receive your antibody 4. Submit your review 5. Receive your 30% off your next order Get Your Free Samples in 5 Easy Steps
- 8. Follow Us for Weekly Independent Validation Reviews @StJohnsLabsSt John's Laboratory Ltd St John's Laboratory Ltd St John's Laboratory Ltd http://www.slideshare.net/stjohnslabs