This chapter discusses the challenges of evidence collection and handling in cyber investigations, emphasizing the importance of applying proper procedures to ensure the integrity of digital evidence. It outlines the roles and responsibilities of investigators, the varying international legal considerations regarding digital evidence, and the need for collaboration with organizations that set standards for handling this type of evidence. Key tasks include identifying, collecting, preserving, and analyzing digital data while adhering to legal principles, especially when operating in a multi-jurisdictional context.