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The construction of a spectrophotometer involves several key components, each playing a vital role in the overall functioning of the instrument. The first critical component is the light source. The choice of the light source depends on the range of wavelengths needed for analysis. For ultraviolet (UV) light, typically a deuterium lamp is used, while tungsten filament lamps are commonly used for the visible light range. In some advanced spectrophotometers, xenon lamps or other broad-spectrum sources may be used to cover a wider range of wavelengths. The light emitted from the source is then directed toward a monochromator, which isolates the desired wavelength of light from the full spectrum emitted by the lamp. Monochromators generally consist of a prism or a diffraction grating, which disperses the light into its component wavelengths. By rotating the monochromator, the instrument can select and pass a narrow band of wavelengths to the sample, ensuring that only light of the desired wavelength reaches the sample compartment.
The sample is typically held in a cuvette, a small transparent container made of quartz, glass, or plastic, depending on the wavelength range of interest. Quartz cuvettes are used for UV measurements since they do not absorb UV light, while plastic or glass cuvettes are sufficient for visible light applications. The path length of the cuvette, usually 1 cm, is a critical parameter because it influences the absorbance readings according to the Beer-Lambert law. Once the monochromatic light passes through the sample, it emerges with reduced intensity due to absorption by the sample. The transmitted light is then collected by a photodetector, which converts the light signal into an electrical signal. This electrical signal is proportional to the intensity of the transmitted light and is processed by the instrument’s electronics to calculate absorbance or transmittance values. These values are then give
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pharmacology.pdffffffffffffffffffffffffffanooshaazam312 Pulmonary delivery of biologics (insulin, vaccines, mRNA)
Definition and Purpose
Pulmonary Delivery: Involves administering biologics directly to the lungs via inhalation.
Goal: To achieve rapid absorption into the bloodstream, enhance bioavailability, and improve therapeutic outcomes.
Types of Biologics
• Insulin: Used for diabetes management; inhaled insulin can provide a non-invasive alternative to injections.
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Advantages
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Gene therapy via inhalation
Definition and Purpose
• Gene Therapy: A technique that involves introducing, removing, or altering genetic material within a patient’s cells to treat or prevent disease.
• Inhalation Delivery: Administering gene therapies directly to the lungs through inhalation, targeting respiratory diseases and conditions.
Mechanism of Action
• Aerosolized Vectors: Utilizes viral or non-viral vectors (e.g., liposomes, nanoparticles) to deliver therapeutic genes to lung cells.
• Cell Uptake: Once inhaled, the vectors penetrate the alveolar epithelium and deliver genetic material to target cells.
Advantages
• Localized Treatment: Direct delivery to the lungs can enhance therapeutic effects while minimizing systemic side effects.
• Non-Invasive: Inhalation is less invasive than traditional injection methods, improving patient compliance.
• Rapid Onset: Potential for quicker therapeutic effects due to direct absorption in the pulmonary system.
Personalized inhaler systems with sensors
• Smart Inhalers: Devices with sensors that track usage and technique.
• Real-Time Monitoring: Connect to apps for data on adherence and inhalation patterns.
• Tailored Treatment: Adjusts medication based on individual usage data.
• Patient Engagement: Provides feedback and reminders to empower self-management.
• Improved Outcomes: Enhances adherence and reduces exacerbations in respiratory conditions.
• Future Potential: May integrate with other health data and use AI for predictive insights.
Sustained-Release Nano Formulations
Definition: Nanoscale drug delivery systems that release therapeutic agents over an extended period.
Components: Made from polymers, lipids, or inorganic materials that encapsulate drugs.
Mechanism:
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Dental_Instruments_Presentation_Updated (3).pptxDental_Instruments_Presentation_Updated Dental_Instruments_Presentation_Updated
- 1. DENTAL INSTRUMENTS Student ID university
- 2. INTRODUCTION Dentists use various instruments to diagnose, treat, and maintain oral health.These tools help in examination, cleaning, extraction, and restoration.
- 3. MOUTH MIRROR Used to provide indirect vision and reflect light inside the mouth.
- 4. DENTAL EXPLORER (PROBE) Helps detect cavities, calculus, and tooth irregularities. • Sickle-shaped. • Detects decay and checks bifurcation. • Identifies carious regions. • Smooth probe movement means stains, not decay—no filling needed.
- 5. PERIODONTAL PROBE Measures pocket depth to assess gum health and detect periodontal disease. • To hold cotton roll. • To hold different material. • To transfer any material in and out of cavity
- 6. SCALER Removes plaque and tartar from teeth surfaces .
- 7. CURETTE Used for deep cleaning below the gumline to prevent periodontal disease .
- 8. ULTRASONIC SCALER Uses vibrations and water spray to remove plaque and calculus efficiently .
- 9. EXCAVATOR Removes decayed tooth material before placing a filling .
- 10. COMPOSITE PLACEMENT INSTRUMENT Used to place and shape composite fillings .
- 11. DENTAL FORCEPS Used for tooth extractions by gripping and removing teeth .
- 12. ENDODONTIC FILES Clean and shape root canals during root canal treatment .
- 13. IMPRESSIONTRAYS Hold impression material to create molds of teeth and gums .
- 14. CONCLUSION Dental instruments are essential for oral health. Proper use and sterilization ensure effective treatments .