Fungal culture, growing of fungi ,preservation technique,# lipophilization

This document discusses fungal culture methodology in the laboratory. It explains that growing fungi in pure culture is necessary for detection, isolation, identification, quantification, multiplication, and utilization of fungi. Pure cultures are also important for genetic resource conservation, development studies, industrial applications, and research programs. The document then describes several techniques for preserving fungal cultures, including periodic transfer, sterile water, sterile mineral oil, sterile soil/sand/silica gel, lyophilization, and their advantages and disadvantages. It emphasizes that preservation is important as fungal cultures are genetic resources.