Group-6 Acid Base Titration.pptx
- 1. WELCOME
- 2. Presentation submitted by: Shoaib Saikat Roll no: 18 BIO 013 Antar Ranjan Karmakar Roll no: 19 BIO 001 Anup Roy Roll no: 19 BIO 004 Presentation submitted to: Md. Hasib Lecturer Department of Biochemistry and Biotechnology, University Of Barisal Topic: Acid Base Titration and Suitable Indicators
- 3. Acid Base 𝐻+ 𝑖𝑜𝑛 𝑂𝐻− 𝑖𝑜𝑛 Indicators substances solutions change color changes in pH Aqueous solutions Aqueous solutions Acid and Base
- 4. Titration technique a solution of known concentration Determine the concentration of an unknown solution Titration Titrant Titrand Acid – Base titration method of quantitative analysis determining the concentration of an acid or base neutralizing it with a standard solution of base or acid known concentration Acid – Base Titration
- 5. PRINCIPLE Acid Acid + Base = Salt + Water Base 01 02 03 𝑝𝐻 𝐶ℎ𝑎𝑛𝑔𝑒 𝐼𝑛𝑑𝑖𝑐𝑎𝑡𝑜𝑟 ↓ 𝑝𝐻 7 𝑁𝑒𝑢𝑡𝑟𝑎𝑙 ↓ 𝐶𝑜𝑙𝑜𝑟 𝑐ℎ𝑎𝑛𝑔𝑒 Nutralization Reaction
- 6. Example of A Neutralization Reaction 𝐻𝐶𝑙(𝑎𝑞) + 𝑁𝑎𝑂𝐻(𝑎𝑞) → 𝑁𝑎𝐶𝑙(𝑎𝑞) + 𝐻2𝑂(𝑙) NaOH HCl → 𝐻+ + 𝐶𝑙− → 𝑁𝑎+ + 𝑂𝐻− NaOH HCl + → NaOH HCl +
- 7. Apparatus Used In Titration Acid Base Indicator Burett e Clamp Stan d
- 8. Base With Indicato r Apparatus Used InTitration Acid Base Indicator
- 9. Base Titration Acid Acid′s Concentration, 𝑆𝐴 = 1 𝑀 Base’s Volume, 𝑉𝐵 = 10 𝑚𝐿 Titrant = HCl Titrand = NaOH
- 10. Acid Base Color Change at pH 7 Acid Acid′s Concentration, 𝑆𝐴 = 1 𝑀 Base’s Volume, 𝑉𝐵 = 10 𝑚𝐿 Titrant = HCl Titrand = NaOH Titration
- 11. Acid Base Calculation Acid Acid′s Concentration, 𝑆𝐴 = 1 𝑀 Base’s Volume, 𝑉𝐵 = 10 𝑚𝐿 Base Initial reading = 10 Final reading = 40 Titrant = HCl Titrand = NaOH
- 12. Calculation Acid′s Concentration, 𝑆𝐴 = 1 𝑀 Initial reading , 𝑉1 = 10 Final reading , 𝑉2 = 40 Titrant = HCl Titrand = NaOH Used Acid’s Volume, 𝑉𝐴 = (𝑉2−𝑉1) = (40 − 10) 𝑚𝐿 = 30 𝑚𝐿 Base’s Volume, 𝑉𝐵 = 10 𝑚𝐿
- 13. 𝑎 × 𝑉𝐴 × 𝑆𝐴 = 𝑏 × 𝑉𝐵 × 𝑆𝐵 a = The number of 𝐻+ 𝑖𝑜𝑛𝑠 𝑡ℎ𝑒 𝐴𝑐𝑖𝑑 𝑐𝑎𝑛 𝑑𝑜𝑛𝑎𝑡𝑒 𝑖𝑛 𝑟𝑒𝑎𝑐𝑡𝑖𝑜𝑛 b = The number of 𝐻+𝑖𝑜𝑛𝑠 𝑡ℎ𝑒 𝐵𝑎𝑠𝑒 𝑐𝑎𝑛 𝑎𝑐𝑐𝑒𝑝𝑡 𝑖𝑛 𝑟𝑒𝑎𝑐𝑡𝑖𝑜𝑛 The Equation, 𝑎 × 𝑉𝐴 × 𝑆𝐴 = 𝑏 × 𝑉𝐵 × 𝑆𝐵 ⇒ 𝑆𝐵 = 𝑎 × 𝑉𝐴 × 𝑆𝐴 𝑏 × 𝑉𝐵 ⇒ 𝑆𝐵 = 1 × 30 × 1 1 × 10 ⇒ 𝑆𝐵 = 3 𝑀 Titrant = HCl Titrand = NaOH a = 1 b = 1
- 14. Indicators Indicator Weak Acid / Weak Base HIn 𝐼𝑛− HIn= Non-ionized 𝐼𝑛− = Ionized
- 15. Case – 1: Strong Acid – Strong Base HIn 𝐼𝑛− Ionized Non-ionized Methyl Red Start Point End Point HIn 𝐼𝑛− Ionized Non-ionized Cresol Red Start Point End Point All Indicators are suitable
- 16. Case – 2: Strong Acid – Weak Base HIn 𝐼𝑛− Ionized Non-ionized Methyl Red Start Point End Point
- 17. Case – 3: Weak Acid – Strong Base HIn 𝐼𝑛− Ionized Non-ionized Start Point End Point Cresol Red
- 18. Case – 4: Weak Acid – Weak Base Weak Acid Weak Base No Suitable Indicators pH Change ↓ Too Gradual
- 19. Titration Curve of a Strong Acid – Strong Base The regions over which the indicators methyl red and phenolphthalein change color along the steep portion of thecurve, they can be used to monitor the equivalence point ofthe titration. Thymol blue, on theother hand, cannot be used forthe same purpose because thecolor change does not match thesteep portion of the titration curve
- 20. The pH range the indicator changes the acid color to the base color
- 21. Chemists use indicators in their analytical work to test substances for their acidity.Also to know the point when an acid neutralises a base. Indicators are used in soil testing. The soil can be slightly acidic or basic. To usean indicator, moisten the soil with a little bit of water and add universal indicator(UI). Then place some white barium sulfate powder on the surface of the soil. Thewhite power absorbs the soil water and the indicator colour can be seen against the white background. Excess soil acidity can be fixed by adding basic substances such as lime. Indicators are used to measure the acidity of water in a swimming pool. If the water is too acidic, it will cause irritations to the eyes and skin. If the water is too basic, green algal scum will grow in the pool. Indicators are used in waste water monitoring. Ensuring alkaline solutions are neutralised before discharged into sewer. Indicators are used in testing water in aquariums. Fish are sensitive to the pH of water so it must be controlled and maintained. Acid-Base indicators In Everyday uses:
Editor's Notes
- #4: THOSE SUBSTANCES THAT PRODUCE ARE substances WHOSE solutions change color DUE TO changes in pH
- #5: IS A------------FOR---------------BY---------------OF
- #6: IF WE ADD ACID AND BASE TOGETHER THEY NEUTRILIZE EACH OTHER BY REACTION AND PRODUCE SALT AND WATER. THIS REACTION IS CALLED NEUTRILIZATION REACTION NOW IN CASE IF WE HAVE USED INDICATOR IN THE ACID OR IN THE BASE, THE COLOR WOULD CHANGE AS THE pH CHANGES TO 7 WHICH IS NEUTRAL PH