Multiple Alignment Sequence using Clustal Omega/ Shumaila Riaz
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The document discusses multiple sequence alignment (MSA), highlighting its importance in molecular biology for aligning biological sequences like protein and DNA. It outlines different types of alignment methods, including progressive and iterative refinement, and mentions various tools used for MSA, such as Clustal Omega. The document also provides a step-by-step guide for performing MSA using Clustal Omega and accessing protein databases.
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Multiple Alignment Sequence using Clustal Omega/ Shumaila Riaz
- 1. Presented by: Shumaila Riaz Roll #: 0827-BH-Z-18 Instructor : Dr. Irfana Liaqat
- 2. Multiple Sequence Alignment Alignment of three or more biological sequences of Protein, DNA, RNA of similar length Types Progressive Alignment Tree Alignment Star Alignment Iterative Refinement Method Tools for MSA Clustal W, Clustal W2, Clustal Omega, T- Coffee, MUSLE
- 3. Clustal Omega Widely used in molecular biology Works by progressive alignment method Most closely related sequences are aligned first, and then additional sequences and group of sequences are added Produce phylogenetic tree
- 4. Steps for Multiple alignment of protein by Clustal Omega NCBI All database (Protein) Name of Protein FASTA Sequence Run Blast Select any 6 sequences Download FASTA sequences Insert in Clustal Omega site Result
- 8. Accession Number: Identifier given to DNA or Protein
- 15. Thank You