Node.js is a JavaScript runtime built on Chrome's V8 JavaScript engine. It allows JavaScript to be run on the server-side instead of in the browser. Node.js uses non-blocking I/O and event looping which makes it lightweight and efficient for data-intensive real-time applications. The Node Package Manager (npm) allows Node.js applications to install additional packages and dependencies. Express.js is a popular web application framework for Node.js that provides features for building web applications and APIs.