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Title: Discuss plant breeding techniques used in self-
 pollinated plants v/s those used in cross-pollinated
                       plants?




Name: Babooa Anusha
Plant breeding is the art and science of improving the genetic makeup of plants in relation to
   their economic use for the mankind. Its continuous aim is to improve the quality, diversity and
   performance of agricultural, horticultural crops. The objective of plant breeding is to develop
   plant that is better adapted to human needs. Various approaches that are used for the genetic
   improvement of crop plants are referred to as plant breeding techniques. The choice of breeding
   techniques mainly depends on the mode of pollination, mode of reproduction, gene action and
   breeding objective of crop species.

   A plant breeder tends to divide crop plants species into two groups: self-pollinating plants and
   cross pollinating plants.

In self-pollinating plants, the
pollen grains are transferred to
the stigma of the same flower or a
different flower in the same plant.

A self-pollinating plant is capable
of fertilizing itself and tends to be
highly homozygous because all of
its genes come from the same
parent. Example of self pollinated
plants is Rice, Wheat, Tomato,
Okra, Peanut, Potato, etc. Broadly,
there is two ways whereby self
pollination can occur.
However, if the pollen grains are
transferred to a flower in another
plant of the same kind, it is called
cross-pollination.

A cross-pollinated plant cannot
fertilize by itself and is likely to be
highly heterozygous. These
species include corn, rye, alfalfa,
clover as well as fruits, nuts and
vegetables.




    Breeding methods for self-pollinating plants are different to some extent from those for cross-
    pollinating ones. The main breeding methods that will be discussed in this report are as follows:

    Commonly used methods known as                    Rarely used methods known as
            general breeding method                              special breeding method

    Plant Introduction                                Mutation breeding
    Selection( pureline ;mass selection; progeny)     polyploidy
    Hybridization (pedigree; bulk,single seed         Transgenic breeding
    descent; backcross)
    Heterosis


    Recurrent selection, multiline varieties and synthetic varieties are mainly used for crop
    improvement.

        1. Plant introduction
           It is introducing a plant into new regions from its growing locality. It is the easiest and
           simplest method. Proper management and acclimatization is very important to prevent
           losses. Quarantine has to play an important role in introduction to ensure that the
           material which is to be introduced should not carry pest& diseases with it. It can be
           used in both self and crossed pollinated plants.
Different geographical regions




                                       Different soil type




        2. Selection

                                                        A. Mass selection

                                            It is the oldest & easiest method of selection and is useful in self-
                                           pollinated species and rarely in cross-pollinated species. It is based
                                           on the ability to recognize desirable or undesirable traits in plants of
                                           a population. The varieties developed through mass selection are
                                           more widely adopted than pure lines though they are not uniform. It
                                           takes about 8 years to produce a variety by this method. Steps
                                           involves: Eliminating undesirable plants as they are grown in the
                                           field (negative mass selection); Tag desirable plants at maturity
                                           (positive mass selection); Select best individuals and mix seed.



B. Pure line selection

It is a method in which a new variety is
developed by selection of a single best plant
progeny among traditional varieties or land
races. It is applied mostly to self-pollinated
crops. It is also used sometimes in cross-
pollinated species for development of inbred
lines. Steps involves: Selection of superior
individual plants (200-3000 plants); visual
C. Progeny selection
          It is used extensively in cross-pollinated species. Plant are selected on basis of
          phenotypic superiority and allowed to open-pollinate. Seeds are harvested
          separately and progeny rows are grown from the selected plant. This process is
          repeated till superior families are selected to produce a new variety.




3. Hybridization/ Heterosis
   The mating of two lines of different genotype is known as hybridization. This method is
   used in both cross & self-pollinated crops. However, it is common in cross-pollinated
   and rare in self-pollinated species. Out crossing (crossing between genetically different
   plant) in cross-pollinate crops often results in heterosis/hybrid vigour. Conversely, cross-
   pollinated plants exhibit inbreeding depression, in the form of small size, poor vigour
   which is contrary to self-pollinated species that do not exhibit inbreeding depression,
   but may exhibit considerable heterosis.
Repeated inbreeding                                    Repeated inbreeding




                                         Inbred A          Inbred B



                                                          F1 A B




A. Pedigree selection
   It is a widely used method of                    A B
   breeding self-pollinated species                                      F1
   (and even cross-pollinated
   species such as crops produced
   as hybrids). Detailed records of                                      F2
   the origin of the selected lines
   are maintained. It produces new
   cultivars faster than mass                                            F3
   selection. In self-pollinated crop,
   it is used to release new
   varieties. In cross-pollinated                                        F4
   crops, it is used to develop
   inbred lines.
                                                                          Fn
B. Bulk method
                                                                             This method can handle
                                                                             segregating generations, in
                                                                             which F2 and subsequent
                                                                             generations are harvested in
                                                                             bulk to grow the next
                                                                             generation. At the end of
                                                                             bulking period, individual
                                                                             plant selection and evaluation
                                                                             is carried out in the similar
                                                                             way as in pedigree method.
                                                                             This method is used in self-
                                                                             pollinated plant species.




   C. Single seed descent

It is a method used in self-pollinated species. It is a modification of
the bulk method. Instead of bulking a whole seed lot of selected
plant, a single seed is selected randomly from each selected plant to
make bulk.
C. Backcross breeding                                                    Well-adapted cultivar

It conserves all good characteristics of a popular
adapted variety and incorporates a desirable
character from another variety. A cross between a
hybrid and one of its parents is known as backcross.
It is applicable to both self and cross-pollinated
                                                            Cultivar with too
species. This method is also used for development of
                                                            many wild
multilines and transfer of male sterility.
                                                            characteristics



                                                                                       New




                                                                      4. Recurrent selection

                                                                  It can be broadly defined as the selection of
                                                                  desirable plants from a population followed by
                                                                  recombination of the selected plants to form a
                                                                  new variety. It is a refined version of the mass
                                                                  selection procedure and is used mainly in
                                                                  cross-pollinated species.




         5. Synthetic and multi line varieties
            Synthetic varieties are developed in cross-pollinated species. It is produces by crossing in
            all combination a number of lines that combine well with each other. These varieties have
            been of great value in the breeding for cross-pollinated crops whereby pollination control
            is difficult. The maize program CIMMYT places a considerable emphasis on synthetic
            varieties.
            Multiline varieties are a mixture of several pure lines of similar phenotype (height, seed
            color) but have different genes for the character under consideration. These varieties are
            developed in self-pollinated crops.
5. Mutation breeding

                                                     Inducing desirable mutations and exploiting them for crop
                                                     improvement is known as mutation breeding. It is more
                                                     valuable in self than cross-pollinated crops. It may occur
                                                     directly during cell division or by exposing the plant to
                                                     irradiation or chemical agents.




   6. Polyploidy
                                                                  Normal cell division
A cell is a polyploid if it has at least
twice the normal number of
chromosomes. It can occur spontaneously
or can be induced using chemicals such
as colchicines. The plants are larger                                              Colchicines         2 diploid
                                           diploid
compared to those having normal number                                             dissolves           cells
of chromosomes. However, it is rarely                                              spindles
used in both self & cross-pollinated
                                                         tetraploid
crops.




                                                          7. Transgenic breeding
                                                             It serves to introduce gene sequences for
                                                             expression of a desired trait. It is applicable to
                                                             both types of crop species. This method is used
                                                             to solve specific problems which cannot be
                                                             solved by conventional breeding techniques.
Plant breeding has several useful applications in the improvement of crop plants. This has
increased the productivity and quality of the plant we grow for food, feed and fiber. However, it
has certain undesirable effects. Conventional plant breeding can no longer sustain the global
demand with the increasing population, decline in agricultural resources such as land and water.
Thus, new crop improvement technologies should be developed and utilized.



References:

       Principles of plant breeding R.W.WALLARD. December 1960
       Agricultural biotechnology, international service for the acquisition of
       AGRI_BIOTECH application, Aug 2010.pdf
       Breeding self-pollinated species.pdf
       FIBL Dossier, plant breeding techniques, No2 September 2001, 1st
       edition.pdf
       EAMCET_QR_botany_SR_botany_11 crop improvement .PDF
       Department of genetics and plant breeding, GBPR 211.pdf

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plant breeding techniques used in self-pollinated plants v/s those used in cross-pollinated plants

  • 1. Title: Discuss plant breeding techniques used in self- pollinated plants v/s those used in cross-pollinated plants? Name: Babooa Anusha
  • 2. Plant breeding is the art and science of improving the genetic makeup of plants in relation to their economic use for the mankind. Its continuous aim is to improve the quality, diversity and performance of agricultural, horticultural crops. The objective of plant breeding is to develop plant that is better adapted to human needs. Various approaches that are used for the genetic improvement of crop plants are referred to as plant breeding techniques. The choice of breeding techniques mainly depends on the mode of pollination, mode of reproduction, gene action and breeding objective of crop species. A plant breeder tends to divide crop plants species into two groups: self-pollinating plants and cross pollinating plants. In self-pollinating plants, the pollen grains are transferred to the stigma of the same flower or a different flower in the same plant. A self-pollinating plant is capable of fertilizing itself and tends to be highly homozygous because all of its genes come from the same parent. Example of self pollinated plants is Rice, Wheat, Tomato, Okra, Peanut, Potato, etc. Broadly, there is two ways whereby self pollination can occur.
  • 3. However, if the pollen grains are transferred to a flower in another plant of the same kind, it is called cross-pollination. A cross-pollinated plant cannot fertilize by itself and is likely to be highly heterozygous. These species include corn, rye, alfalfa, clover as well as fruits, nuts and vegetables. Breeding methods for self-pollinating plants are different to some extent from those for cross- pollinating ones. The main breeding methods that will be discussed in this report are as follows: Commonly used methods known as Rarely used methods known as general breeding method special breeding method Plant Introduction Mutation breeding Selection( pureline ;mass selection; progeny) polyploidy Hybridization (pedigree; bulk,single seed Transgenic breeding descent; backcross) Heterosis Recurrent selection, multiline varieties and synthetic varieties are mainly used for crop improvement. 1. Plant introduction It is introducing a plant into new regions from its growing locality. It is the easiest and simplest method. Proper management and acclimatization is very important to prevent losses. Quarantine has to play an important role in introduction to ensure that the material which is to be introduced should not carry pest& diseases with it. It can be used in both self and crossed pollinated plants.
  • 4. Different geographical regions Different soil type 2. Selection A. Mass selection It is the oldest & easiest method of selection and is useful in self- pollinated species and rarely in cross-pollinated species. It is based on the ability to recognize desirable or undesirable traits in plants of a population. The varieties developed through mass selection are more widely adopted than pure lines though they are not uniform. It takes about 8 years to produce a variety by this method. Steps involves: Eliminating undesirable plants as they are grown in the field (negative mass selection); Tag desirable plants at maturity (positive mass selection); Select best individuals and mix seed. B. Pure line selection It is a method in which a new variety is developed by selection of a single best plant progeny among traditional varieties or land races. It is applied mostly to self-pollinated crops. It is also used sometimes in cross- pollinated species for development of inbred lines. Steps involves: Selection of superior individual plants (200-3000 plants); visual
  • 5. C. Progeny selection It is used extensively in cross-pollinated species. Plant are selected on basis of phenotypic superiority and allowed to open-pollinate. Seeds are harvested separately and progeny rows are grown from the selected plant. This process is repeated till superior families are selected to produce a new variety. 3. Hybridization/ Heterosis The mating of two lines of different genotype is known as hybridization. This method is used in both cross & self-pollinated crops. However, it is common in cross-pollinated and rare in self-pollinated species. Out crossing (crossing between genetically different plant) in cross-pollinate crops often results in heterosis/hybrid vigour. Conversely, cross- pollinated plants exhibit inbreeding depression, in the form of small size, poor vigour which is contrary to self-pollinated species that do not exhibit inbreeding depression, but may exhibit considerable heterosis.
  • 6. Repeated inbreeding Repeated inbreeding Inbred A Inbred B F1 A B A. Pedigree selection It is a widely used method of A B breeding self-pollinated species F1 (and even cross-pollinated species such as crops produced as hybrids). Detailed records of F2 the origin of the selected lines are maintained. It produces new cultivars faster than mass F3 selection. In self-pollinated crop, it is used to release new varieties. In cross-pollinated F4 crops, it is used to develop inbred lines. Fn
  • 7. B. Bulk method This method can handle segregating generations, in which F2 and subsequent generations are harvested in bulk to grow the next generation. At the end of bulking period, individual plant selection and evaluation is carried out in the similar way as in pedigree method. This method is used in self- pollinated plant species. C. Single seed descent It is a method used in self-pollinated species. It is a modification of the bulk method. Instead of bulking a whole seed lot of selected plant, a single seed is selected randomly from each selected plant to make bulk.
  • 8. C. Backcross breeding Well-adapted cultivar It conserves all good characteristics of a popular adapted variety and incorporates a desirable character from another variety. A cross between a hybrid and one of its parents is known as backcross. It is applicable to both self and cross-pollinated Cultivar with too species. This method is also used for development of many wild multilines and transfer of male sterility. characteristics New 4. Recurrent selection It can be broadly defined as the selection of desirable plants from a population followed by recombination of the selected plants to form a new variety. It is a refined version of the mass selection procedure and is used mainly in cross-pollinated species. 5. Synthetic and multi line varieties Synthetic varieties are developed in cross-pollinated species. It is produces by crossing in all combination a number of lines that combine well with each other. These varieties have been of great value in the breeding for cross-pollinated crops whereby pollination control is difficult. The maize program CIMMYT places a considerable emphasis on synthetic varieties. Multiline varieties are a mixture of several pure lines of similar phenotype (height, seed color) but have different genes for the character under consideration. These varieties are developed in self-pollinated crops.
  • 9. 5. Mutation breeding Inducing desirable mutations and exploiting them for crop improvement is known as mutation breeding. It is more valuable in self than cross-pollinated crops. It may occur directly during cell division or by exposing the plant to irradiation or chemical agents. 6. Polyploidy Normal cell division A cell is a polyploid if it has at least twice the normal number of chromosomes. It can occur spontaneously or can be induced using chemicals such as colchicines. The plants are larger Colchicines 2 diploid diploid compared to those having normal number dissolves cells of chromosomes. However, it is rarely spindles used in both self & cross-pollinated tetraploid crops. 7. Transgenic breeding It serves to introduce gene sequences for expression of a desired trait. It is applicable to both types of crop species. This method is used to solve specific problems which cannot be solved by conventional breeding techniques.
  • 10. Plant breeding has several useful applications in the improvement of crop plants. This has increased the productivity and quality of the plant we grow for food, feed and fiber. However, it has certain undesirable effects. Conventional plant breeding can no longer sustain the global demand with the increasing population, decline in agricultural resources such as land and water. Thus, new crop improvement technologies should be developed and utilized. References: Principles of plant breeding R.W.WALLARD. December 1960 Agricultural biotechnology, international service for the acquisition of AGRI_BIOTECH application, Aug 2010.pdf Breeding self-pollinated species.pdf FIBL Dossier, plant breeding techniques, No2 September 2001, 1st edition.pdf EAMCET_QR_botany_SR_botany_11 crop improvement .PDF Department of genetics and plant breeding, GBPR 211.pdf