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Muhammad Zeeshan Nazar
M.Phil Agriculture Entomology
mzeeshan_93@yahoo.com
Phytohormones
• Naturally occurring organic compounds
• Relatively low molecular weight
• Active in small concentrations
•Synthesized at one site and translocated to the site of
action, where they regulate the activities of plant.
Plant Growth Regulators
 Chemicals (natural or synthetic) which show
hormonal effects to plants.
Major Phytohormones
 Auxin
 Cytokinin
 Gibberellin
 Abscisic acid
 Ethylene
 Cycocel
 Maleic Hydrazide
Growth promoters
Growth Retardants
Growth Inhibitors
Auxin
 Darwin noted tropistic response of grass tip coleoptile
in 1897
 Went found these substances in Avena coleoptile.
 These substances become known as Auxins and
necessary for growth
 Found in growing regions such as terminal and lateral
buds, elongating internodes and the young embryo in
its developing stage inside the seed.
 Responsible for cell elongation and cell division
 They are translocated through the vascular bundles to
the other parts of the plant, especially from apical to
basal regions.
 The accumulation of auxins in the terminal bud
inhibits the growth of lateral buds, which can be
avoided by prunning.
Natural Auxin
 Indole acetic acid (IAA)
Synthetic Auxin
 indole‐3‐butyric acid (IBA)
 ‐naphthalene acetic acid (NAA)
Uses of Auxins
 Rooting of cuttings of various fruit species e.g
mulberry, pomegranate, fig, grapes and olives are
treated with IBA and NAA for early initiation of
adventitious roots.
 Killing of weeds e.g 2,4 D is used for killing of broad
leaf weeds.
 Micropropagation of plant species
 Setting of fruits in tomato, by spraying of auxin 4-
Chlorophenoxyacetic acid (4-CPA) before flowering.
 Pre-Harvest Drop Control by spray of NAA in
oranges
Cytokinin
 It was identified in 1955 and was called kinetin
 It was an active cell division stimulator
Natural cytokinin
 –Zeatin obtained from maize seed and coconut
Synthetic cytokinin
 –Kinetin
 –benzyladenine (BA or BAP)
It is present in embryos, germinating seeds, in early
stages of developing fruits and in meristematic tissues.
Uses of cytokinins
 To stimulate shoot development
 Delaying senescence
 Accelerating bud growth
Gibberellin
 Gibberellin was discovered in 1930, during studies on
fungal disease of rice, which caused excessive growth.
 The fungus which produce these substances was known
as Gibberella fugikuroi.
 So the new substance was known as gibberellin
 Gibberellin stimulate shoot elongation and control ger
mination and dormancy
 Present in the apices of shoots and leaf primordia of the
plant, in embryo and cotyledons of the immature seed
and fruit tissues.
Uses of Gibberellins
 Enhancing seed germination and seedling growth
 Eliminating cold treatment
 Initiate male flowers in cucumber
Abscisic acid
Plant growth hormone that plays a role in
 Plant stress,
 Controlling water relations,
 Embryo development,
 Germination and dormancy
Found in various plant tissues; cotton fruits, leaves of
plants under water stress, fruits of rose.
It is believed to be anti-auxin and anti-gibberellin in
growth promoting actions.
Ethylene
Natural plant growth hormone involved with fruit
maturity and stimulation of adventitious roots
Ethylene in its gaseous form spreads easily through the
plant and affects it even in small quantities.
Produced in the meristematic tissues of the plant,
germinating seeds, withering flowers, maturing and
ripened fruits and in injured plant tissues.
Synthetic product “Ethephon” produces good amount of
ethylene, when applied to plants.
Uses
 Inducing fruit maturity
 Initiating flowers
 Altering the sex of flowers
 Changing the green color of citrus fruits
 Abscission inducing effects

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Plant Growth Regulators

  • 2. Phytohormones • Naturally occurring organic compounds • Relatively low molecular weight • Active in small concentrations •Synthesized at one site and translocated to the site of action, where they regulate the activities of plant.
  • 3. Plant Growth Regulators  Chemicals (natural or synthetic) which show hormonal effects to plants.
  • 4. Major Phytohormones  Auxin  Cytokinin  Gibberellin  Abscisic acid  Ethylene  Cycocel  Maleic Hydrazide Growth promoters Growth Retardants Growth Inhibitors
  • 5. Auxin  Darwin noted tropistic response of grass tip coleoptile in 1897  Went found these substances in Avena coleoptile.  These substances become known as Auxins and necessary for growth  Found in growing regions such as terminal and lateral buds, elongating internodes and the young embryo in its developing stage inside the seed.  Responsible for cell elongation and cell division
  • 6.  They are translocated through the vascular bundles to the other parts of the plant, especially from apical to basal regions.  The accumulation of auxins in the terminal bud inhibits the growth of lateral buds, which can be avoided by prunning.
  • 7. Natural Auxin  Indole acetic acid (IAA) Synthetic Auxin  indole‐3‐butyric acid (IBA)  ‐naphthalene acetic acid (NAA)
  • 8. Uses of Auxins  Rooting of cuttings of various fruit species e.g mulberry, pomegranate, fig, grapes and olives are treated with IBA and NAA for early initiation of adventitious roots.  Killing of weeds e.g 2,4 D is used for killing of broad leaf weeds.  Micropropagation of plant species  Setting of fruits in tomato, by spraying of auxin 4- Chlorophenoxyacetic acid (4-CPA) before flowering.  Pre-Harvest Drop Control by spray of NAA in oranges
  • 9. Cytokinin  It was identified in 1955 and was called kinetin  It was an active cell division stimulator Natural cytokinin  –Zeatin obtained from maize seed and coconut Synthetic cytokinin  –Kinetin  –benzyladenine (BA or BAP) It is present in embryos, germinating seeds, in early stages of developing fruits and in meristematic tissues.
  • 10. Uses of cytokinins  To stimulate shoot development  Delaying senescence  Accelerating bud growth
  • 11. Gibberellin  Gibberellin was discovered in 1930, during studies on fungal disease of rice, which caused excessive growth.  The fungus which produce these substances was known as Gibberella fugikuroi.  So the new substance was known as gibberellin  Gibberellin stimulate shoot elongation and control ger mination and dormancy  Present in the apices of shoots and leaf primordia of the plant, in embryo and cotyledons of the immature seed and fruit tissues.
  • 12. Uses of Gibberellins  Enhancing seed germination and seedling growth  Eliminating cold treatment  Initiate male flowers in cucumber
  • 13. Abscisic acid Plant growth hormone that plays a role in  Plant stress,  Controlling water relations,  Embryo development,  Germination and dormancy Found in various plant tissues; cotton fruits, leaves of plants under water stress, fruits of rose. It is believed to be anti-auxin and anti-gibberellin in growth promoting actions.
  • 14. Ethylene Natural plant growth hormone involved with fruit maturity and stimulation of adventitious roots Ethylene in its gaseous form spreads easily through the plant and affects it even in small quantities. Produced in the meristematic tissues of the plant, germinating seeds, withering flowers, maturing and ripened fruits and in injured plant tissues. Synthetic product “Ethephon” produces good amount of ethylene, when applied to plants.
  • 15. Uses  Inducing fruit maturity  Initiating flowers  Altering the sex of flowers  Changing the green color of citrus fruits  Abscission inducing effects