PHYTOHORMONES
SEMESTER VI- BOTANY
PLANT PHYSIOLOGY
PAPER VI
P.HARITHA
DL BOTANY
TTWRDC (M)
SANGAREDDY
What do you observe and
infer???
Definition
Characteristics
Classification
Functions of various
hormones
What are Plant
Hormones?
What are different types
of hormones and where
are they produced in
plant?
Plant hormones are “Organic
compounds synthesized in one
part of the plant and translocated
to another part”.
Plant hormones are also
termed as
- Phytohormones
- Plant growth bio regulators
- Growth substances
Endogenously
produced
In less
concentrations
Highly
diffusible
Specific in function
May be stimulatory
or inhibitory in
function
Characteristics of Growth substances
Classification of growth
substances
Auxins Cytokinin
Abscisic
acid
Gibberellins
Ethylene
Brassino-
steroids
Classes of Plant growth regulators :It is accepted
that there are two major classes of plant
hormones:
Auxins
Cytokinin
Gibberellins
Brassinosteroids
Promoters
Ethylene
ABA
Inhibitors
Plant hormones   or growth regulators
Key features of each Growth substance
Isolation Structure
Mode of
action
Physiological
aspects
Auxins
 F.W.Went was first to isolate
Auxins from Avena, Wheat and
Maize.
 Thimman defined Auxin as “ an
organic substance which at
low concentration promote
growth along longitudinal
axis”.
 Auxins occur in all groups of
plants.
 Auxin is made in actively
growing tissue which includes
young leaves, fruits, and
especially the shoot apex,
legume root nodules,
germinating pollen grains
 Made in cytosol of cells
 Transport of auxins is polar
through phloem & parenchyma
by active transport.
 Chemically Auxins are Indole 3
Acetic Acid (IAA)
 The Chemical formula: C10H9NO2
 Molar mass: 175.187 g·mol−1y
 An Auxin is made of
 1. Indole ring
 2. a carboxyl group and
 3. at least 1 carbon atom
between ring and carboxyl group
Natural auxins
Indole Acetaldehyde
Indole Ethanol
Indole Aceto Nitrate
These get oxidized to IAA by various
plant enzymes
Synthetic Auxins
Naphthalene Acetic Acid (NAA)
Indole Butyric Acid (IBA)
2,4,Dichloro Phenoxy Acetic Acid (2,4- D)
2,4,5,Trichloro Phenoxy Acetic Acid (2,4,5-
T)
2 Methyl 4 Chloro Phenoxy Acetic acid)
(MCPA)
Indole 3 Propionic Acid (IPA)IBA & IPA – used as Rooting
hormones
2,4,D & 2,4,5-T used as selective
herbicides
NAA- used in horticulture
practices
Cell enlargement
Cell division
Apical cell dominance and inhibition of lateral buds
Root formation
Tropisms
Flower formation
parthenocarpy
Abscission of leaves and fruits
Weed killers
Sex expression
Physiological effects of Auxins
Plant hormones   or growth regulators
Mode
of
action
DNA replication,
transcription and
translation
Through
ARC
complex –
RNA
synthesis
Loosening of
cell wall
Nucleic acid
synthesis
AUXINS IN AGRI AND HORTICULTURE
 Treating stem cuttings and scions for vegetative propagation.
 control of flowering
 Prevents sprouting of buds
 Defoliation of plants
 Prevent leaf fall
 Thinning of compact fruits
 Weedicide
 Development of parthenocarpic fruits
 Increasing fruit set
 Prevention of premature fruit drop
Gibberllins
 Gibberellins are 4 or 5 ringed
diterpenoids which is made of gibbane
carbon skeleton.
 Synthesis of gibberellins is by
Mevalonic acid pathway along with
terpenes, sterols and carotenoids.
 Formula: C19H22O6
 Molar mass: 346.37 g/mol
 Discovered by Kurosawa in Rice
which was affected by ‘Bakane’
disease caused by fungus
Gibberella fujikuroi
 They are found in all parts of
plant but abundant in seeds and
young leaves.
 50 kinds of Gibberellins are
isolated till now, out of which
GA1 & GA 3-9 common in all
plants
 Where as GA2 found only in
fungus.
 Movement of gibberellins is non
polar
 By diffusion it moves through
xylem and phloem
PHYSIOLOGICAL EFFECTS OF GIBBERELLINS
Effect on dwarf mutants
Bolting and flowering
Substituting cold treatment
Breaking of dormancy
parthenocarpy
Increase in size of leaves, flowers and fruits
Seed germination
Flowering and sex expression
Plant hormones   or growth regulators
Mode
of
action
Stem/cell
elongation
Affect
RNA and
Protein
synthesis
GA binding
Control
Mitotic
cycle
COMMERCIAL USES OF GIBBERLLINS
 GA2, GA3,GA7 used for enhancing Grape cluster and size.
 Production of seedless grapes
 Gibberellins enhance α- amylase in barley, which is used in Beer
industry.
 Promote internodal and stalk length, increase in sugar content in
Sugar cane.
 Flower initiation in certain ornamentals like Poinsettia etc.
 Preharvest spray with gibberellins delays fruit ripening which
help in increased storage period during transport of fruits.
CYTOKININS
 Cytokinins are 6 furfuryl aminopurine-
a derivative of Adenine.
 Synthesis by t-RNA cytokinin
synthesis/ free cytokinin synthesis/
T-DNA related CK synthesis
 Chemical formula C10H13N5O
 Molar weight 219.24 g/mol
 Haberlandt first identified a
substance which would trigger
cell divisions
 Miller isolated and named them
Kinetin.
 They occur in all non vascular
and vascular plants.
 Found in apical meristems,
developing seeds, roots, fruit
and cambial tissues.
 In few pathogenic bacteria and
fungi, influence disease process
in host-parasite relationship.
 Cytokinin occur naturally and
synthetic made too
 Synthesized in young leaves,
fruits, seeds, root tips and
translocated by xylem and sieve
tubes.
Cell division and morphogenesis
Cell enlargement
Breaking seed dormancy
Counter action of apical dominance effect of
enzymes
Delay in senescence
Resistance to adverse effects
Stomatal movements
Development of inflorescence/ enhance fruit set
and size
PHYSIOLOGICAL EFFECTS OF CYTOKININS
Plant hormones   or growth regulators
Mode
of
action
Nucleic acid
metabolism/
Translation
Binding
factors
Stimulate
respiration
Retard/
delay
senescence
ABSCISIC ACID
 ABA is a Sesquiterpenoid, it contains
an asymetric carbon atom which exists
in (+) or (-) entaiomers.
 2 CIS-ABA is active naturally occurring
ABA.
 Formula: C15H20O4
 Molar mass: 264.32 g/mol
 Addicot et al discovered ABA.
 It is a natural growth
inhibiting phytohormone.
 ABA is naturally occurring as
well as synthetic in form.
 ABA & phenolic compounds-
natural inhibitor
 Malic hydrazide, AMO-1618,
Morphactin, Chlorocholine
chloride- synthetic inhibitors.
 ABA is found in all plant
groups except Liverworts and
few algae.
 In Liverworts ABA is
substituted by Lunularic acid.
 ABA as a growth inhibitor controls unending
enlargement of internodes of stems, leaves or
any part of the plant.
 From leaves it gets transported to shoot and
root tips through xylem & phloem.
 Movement is Basipetal.
 ABA is also referred as Anti Auxin/ Anti
Gibberellins/ Anti Cytokinin
 Biosynthesis is by 2 ways- Indirect
Xanthophyll cleavage pathway-
higher plants
 Direct pathway – Isoprenoid pathway-
Fungi
PHYSIOLOGICAL EFFECTS OF ABA
Growth inhibition
Dormancy
Abscission
Seed development and germination
Fruit growth and flowering
Senescence
Stomatal movements
Root geotropism
Plant hormones   or growth regulators
Mode
of
action
Regulate
enzyme
synthesis
Proton and
Potassium
pump
Stomatal
responses
Inhibition at
translational
level
ETHYLENE
 Formula: C2H4
 Molar mass: 28.05 g/mol
 Auxins seem to regulate Ethylene
production.
 Both auxins and ethylene found in
similar parts of plant in high
concentrations
 Discovered by Neljuibow
 A gaseous Phytohormone
 Found in all plants in traces
 Produced in more quantities
in meristematic and growing
regions and nodal parts,
specially produced in large
quantities in fruits
 Non polar transport by
diffusion
 Biosynthetic pathway unclear
 But requires Methionine as a
Precursor.
PHYSIOLOGICAL ASPECTS OF ETHYLENE
Abscission
Ripening
Effect on growth
Degreening
Senescence
Flowering
Dormancy
Plant hormones   or growth regulators
Mode
of
action
Induce
Enzyme
changes
RNA
synthesis
Protein
synthesis
Enzyme –
Receptor
complex
PRACTICAL APPLICATIONS OF ETHYLENE
 ETHREL(2-chloroethyl-phosphonic acid) is a synthetic ethylene
used commercially
 It helps in acceleration of fruit ripening- Banana, Apples
 Induce flowering in Pineapples
 Cause senescence in Tobacco leaves
 Prolongs storage life of fruits
 Regulate fruit or leaf fall in commercial plants at appropriate
time
BRASSIONSTEROIDS  Chemically Polyhydroxy steroids and
has common 5 α-choleston skeleton
and show structural variation in side
chain.
 2 Biosynthetic pathways-Early C6 and
Late C6 oxidation
 Molecular Formula: C28H48O6
 Molecular Weight: 480.7 g/mol
 Regarded as Sixth group of Plant
growth regulators.
 First identified by Mitchell et al
 Discovered in Brassica napus and
later identified in 44 plants
 (37 angiosperms, 5 gymnosperms
and one each in algae and
pteridophyte)
 Show growth promoting activity
 Found in very minute quantities
 Pollen grains and immature seeds
have highest concentration of
brassinosteroids.
 Used in improving yield and growth
of many vegetable yielding plants.
PHYSIOLOGICAL EFFECTS OF BRASSIONSTEROIDS
Growth
Germination
Flowering
Senescence
Stress tolerance to abiotic factors
Resistance
to chilling
Eg. Rice
Low
temperature
stress
Eg. Maize
Tolerance to
high
temperatures
Eg. Wheat
DIFFERENT KINDS OF
ABIOTIC STRESS
COUNTERED BY
BRASSIONSTREROIDS
Resistance
to drought
stress
Eg. Sugar
beet
Normal
germination of
seeds under saline
conditions
Eg. Eucalyptus
Removal of salt
stress
Eg. Seedlings
of Groundnut
Plant hormones   or growth regulators
Mode
of
action
Signal
transduction
Regulate
gene
expression
Genes
associated
with plant
growth
Activation
of kinase
systems
POST GRADUATION
ENTRANCE TEST
AND
HOW TO PREPARE????
Plant hormones   or growth regulators
Plant hormones   or growth regulators
Plant hormones   or growth regulators
Plant hormones   or growth regulators
Plant hormones   or growth regulators
Plant hormones   or growth regulators
BOOKS TO PREPARE FOR PG ENTRANCE EXAMS
Thank you
Acknowlegdements
I would like to give credit to
authors and owners whose
content I used in the
presentation from the open free
internet portal

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Plant hormones or growth regulators

  • 1. PHYTOHORMONES SEMESTER VI- BOTANY PLANT PHYSIOLOGY PAPER VI P.HARITHA DL BOTANY TTWRDC (M) SANGAREDDY
  • 2. What do you observe and infer???
  • 4. What are Plant Hormones? What are different types of hormones and where are they produced in plant?
  • 5. Plant hormones are “Organic compounds synthesized in one part of the plant and translocated to another part”. Plant hormones are also termed as - Phytohormones - Plant growth bio regulators - Growth substances
  • 6. Endogenously produced In less concentrations Highly diffusible Specific in function May be stimulatory or inhibitory in function Characteristics of Growth substances
  • 7. Classification of growth substances Auxins Cytokinin Abscisic acid Gibberellins Ethylene Brassino- steroids
  • 8. Classes of Plant growth regulators :It is accepted that there are two major classes of plant hormones: Auxins Cytokinin Gibberellins Brassinosteroids Promoters Ethylene ABA Inhibitors
  • 10. Key features of each Growth substance Isolation Structure Mode of action Physiological aspects
  • 11. Auxins  F.W.Went was first to isolate Auxins from Avena, Wheat and Maize.  Thimman defined Auxin as “ an organic substance which at low concentration promote growth along longitudinal axis”.  Auxins occur in all groups of plants.  Auxin is made in actively growing tissue which includes young leaves, fruits, and especially the shoot apex, legume root nodules, germinating pollen grains  Made in cytosol of cells  Transport of auxins is polar through phloem & parenchyma by active transport.  Chemically Auxins are Indole 3 Acetic Acid (IAA)  The Chemical formula: C10H9NO2  Molar mass: 175.187 g·mol−1y  An Auxin is made of  1. Indole ring  2. a carboxyl group and  3. at least 1 carbon atom between ring and carboxyl group
  • 12. Natural auxins Indole Acetaldehyde Indole Ethanol Indole Aceto Nitrate These get oxidized to IAA by various plant enzymes Synthetic Auxins Naphthalene Acetic Acid (NAA) Indole Butyric Acid (IBA) 2,4,Dichloro Phenoxy Acetic Acid (2,4- D) 2,4,5,Trichloro Phenoxy Acetic Acid (2,4,5- T) 2 Methyl 4 Chloro Phenoxy Acetic acid) (MCPA) Indole 3 Propionic Acid (IPA)IBA & IPA – used as Rooting hormones 2,4,D & 2,4,5-T used as selective herbicides NAA- used in horticulture practices
  • 13. Cell enlargement Cell division Apical cell dominance and inhibition of lateral buds Root formation Tropisms Flower formation parthenocarpy Abscission of leaves and fruits Weed killers Sex expression Physiological effects of Auxins
  • 15. Mode of action DNA replication, transcription and translation Through ARC complex – RNA synthesis Loosening of cell wall Nucleic acid synthesis
  • 16. AUXINS IN AGRI AND HORTICULTURE  Treating stem cuttings and scions for vegetative propagation.  control of flowering  Prevents sprouting of buds  Defoliation of plants  Prevent leaf fall  Thinning of compact fruits  Weedicide  Development of parthenocarpic fruits  Increasing fruit set  Prevention of premature fruit drop
  • 17. Gibberllins  Gibberellins are 4 or 5 ringed diterpenoids which is made of gibbane carbon skeleton.  Synthesis of gibberellins is by Mevalonic acid pathway along with terpenes, sterols and carotenoids.  Formula: C19H22O6  Molar mass: 346.37 g/mol  Discovered by Kurosawa in Rice which was affected by ‘Bakane’ disease caused by fungus Gibberella fujikuroi  They are found in all parts of plant but abundant in seeds and young leaves.  50 kinds of Gibberellins are isolated till now, out of which GA1 & GA 3-9 common in all plants  Where as GA2 found only in fungus.  Movement of gibberellins is non polar  By diffusion it moves through xylem and phloem
  • 18. PHYSIOLOGICAL EFFECTS OF GIBBERELLINS Effect on dwarf mutants Bolting and flowering Substituting cold treatment Breaking of dormancy parthenocarpy Increase in size of leaves, flowers and fruits Seed germination Flowering and sex expression
  • 21. COMMERCIAL USES OF GIBBERLLINS  GA2, GA3,GA7 used for enhancing Grape cluster and size.  Production of seedless grapes  Gibberellins enhance α- amylase in barley, which is used in Beer industry.  Promote internodal and stalk length, increase in sugar content in Sugar cane.  Flower initiation in certain ornamentals like Poinsettia etc.  Preharvest spray with gibberellins delays fruit ripening which help in increased storage period during transport of fruits.
  • 22. CYTOKININS  Cytokinins are 6 furfuryl aminopurine- a derivative of Adenine.  Synthesis by t-RNA cytokinin synthesis/ free cytokinin synthesis/ T-DNA related CK synthesis  Chemical formula C10H13N5O  Molar weight 219.24 g/mol  Haberlandt first identified a substance which would trigger cell divisions  Miller isolated and named them Kinetin.  They occur in all non vascular and vascular plants.  Found in apical meristems, developing seeds, roots, fruit and cambial tissues.  In few pathogenic bacteria and fungi, influence disease process in host-parasite relationship.  Cytokinin occur naturally and synthetic made too  Synthesized in young leaves, fruits, seeds, root tips and translocated by xylem and sieve tubes.
  • 23. Cell division and morphogenesis Cell enlargement Breaking seed dormancy Counter action of apical dominance effect of enzymes Delay in senescence Resistance to adverse effects Stomatal movements Development of inflorescence/ enhance fruit set and size PHYSIOLOGICAL EFFECTS OF CYTOKININS
  • 26. ABSCISIC ACID  ABA is a Sesquiterpenoid, it contains an asymetric carbon atom which exists in (+) or (-) entaiomers.  2 CIS-ABA is active naturally occurring ABA.  Formula: C15H20O4  Molar mass: 264.32 g/mol  Addicot et al discovered ABA.  It is a natural growth inhibiting phytohormone.  ABA is naturally occurring as well as synthetic in form.  ABA & phenolic compounds- natural inhibitor  Malic hydrazide, AMO-1618, Morphactin, Chlorocholine chloride- synthetic inhibitors.  ABA is found in all plant groups except Liverworts and few algae.  In Liverworts ABA is substituted by Lunularic acid.
  • 27.  ABA as a growth inhibitor controls unending enlargement of internodes of stems, leaves or any part of the plant.  From leaves it gets transported to shoot and root tips through xylem & phloem.  Movement is Basipetal.  ABA is also referred as Anti Auxin/ Anti Gibberellins/ Anti Cytokinin  Biosynthesis is by 2 ways- Indirect Xanthophyll cleavage pathway- higher plants  Direct pathway – Isoprenoid pathway- Fungi
  • 28. PHYSIOLOGICAL EFFECTS OF ABA Growth inhibition Dormancy Abscission Seed development and germination Fruit growth and flowering Senescence Stomatal movements Root geotropism
  • 31. ETHYLENE  Formula: C2H4  Molar mass: 28.05 g/mol  Auxins seem to regulate Ethylene production.  Both auxins and ethylene found in similar parts of plant in high concentrations  Discovered by Neljuibow  A gaseous Phytohormone  Found in all plants in traces  Produced in more quantities in meristematic and growing regions and nodal parts, specially produced in large quantities in fruits  Non polar transport by diffusion  Biosynthetic pathway unclear  But requires Methionine as a Precursor.
  • 32. PHYSIOLOGICAL ASPECTS OF ETHYLENE Abscission Ripening Effect on growth Degreening Senescence Flowering Dormancy
  • 35. PRACTICAL APPLICATIONS OF ETHYLENE  ETHREL(2-chloroethyl-phosphonic acid) is a synthetic ethylene used commercially  It helps in acceleration of fruit ripening- Banana, Apples  Induce flowering in Pineapples  Cause senescence in Tobacco leaves  Prolongs storage life of fruits  Regulate fruit or leaf fall in commercial plants at appropriate time
  • 36. BRASSIONSTEROIDS  Chemically Polyhydroxy steroids and has common 5 α-choleston skeleton and show structural variation in side chain.  2 Biosynthetic pathways-Early C6 and Late C6 oxidation  Molecular Formula: C28H48O6  Molecular Weight: 480.7 g/mol  Regarded as Sixth group of Plant growth regulators.  First identified by Mitchell et al  Discovered in Brassica napus and later identified in 44 plants  (37 angiosperms, 5 gymnosperms and one each in algae and pteridophyte)  Show growth promoting activity  Found in very minute quantities  Pollen grains and immature seeds have highest concentration of brassinosteroids.  Used in improving yield and growth of many vegetable yielding plants.
  • 37. PHYSIOLOGICAL EFFECTS OF BRASSIONSTEROIDS Growth Germination Flowering Senescence Stress tolerance to abiotic factors
  • 38. Resistance to chilling Eg. Rice Low temperature stress Eg. Maize Tolerance to high temperatures Eg. Wheat DIFFERENT KINDS OF ABIOTIC STRESS COUNTERED BY BRASSIONSTREROIDS Resistance to drought stress Eg. Sugar beet Normal germination of seeds under saline conditions Eg. Eucalyptus Removal of salt stress Eg. Seedlings of Groundnut
  • 48. BOOKS TO PREPARE FOR PG ENTRANCE EXAMS
  • 49. Thank you Acknowlegdements I would like to give credit to authors and owners whose content I used in the presentation from the open free internet portal