Role of Bio-Fertilizers in Sustainable Agriculture

Mediterranean Journal of Basic and Applied Sciences (MJBAS)
(Quarterly International Journal), Volume 4, Issue 2, Pages 08-23, April-June 2020
8 | P a g e ISSN (Online): 2581-5059 Website: www.mjbas.com
Role of Bio-Fertilizers in Sustainable Agriculture
Muhammad Salman Akram1
, Muhammad Abubakar Cheema1
, Faisal Nadeem1
, Muhammad Waqas2
, Muhammad
Bilal3
and Muhammad Saeed1
*
1
*Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.
2
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
3
Basic Health Unit Sarki district Muzaffargarh Pakistan.
Correspondence Author Email: muhammadsaeed7360@gmail.com1
*
DOI: 10.46382/MJBAS.2020.4202
Article Received: 09 January 2020 Article Accepted: 16 March 2020 Article Published: 29 April 2020
Introduction
By increasing population of the world the demand for the food is increasing rapidly (1,2). The food demand is
increasing in those developed countries where the land resources are not contributing part in crop production as the
population need in daily bases needs the food requirements.to increase this capacity of land to produce more
production the people first move to the application of chemical fertilizers which increase crop production. But the
long application of chemical fertilizers shows adverse effects on the environment (1,3,4). These chemicals
accumulate in the water and also can the cause of eutrophication (2,3). These chemicals also harm the soil fertility,
decrease Holding capacity of soil, increases the salinity and disparity in soil nutrients (1). So the intention goes
towards the bio fertilizers. These were used to decrease the bad impacts of low fertility on the soil , the impact of
environmental stress and the effect of biotic stress such as pathogens and other microorganisms (5–7). In this aspect
many work has done in this field so many organic fertilizers has been introduced in these years. These work as the
natural stimulators of plant growth and the development of the plants (8–10). Important group of organic fertilizers
were made on the basis of plant growth promoting microorganisms (PGPM). There are many groups of the
microorganism which are beneficial for the plants but the major contribution are of the following three which
considered as important for plants growth: arbuscular mycorrhizal fungi (10,11). Plant growth promoting
rhizobacteria, and nitrogen fixing bacteria that are not considered as PGPR(4,10) the bio fertilizers are the material
that contain living microbes that applied on seed, surfaces of plants or the soil, formation of colonies the
rhizosphere or the interior of the plant, and promote the growth of the plant by increasing the supply of primary
nutrients or giving the availability of these to the host (10,12). The main interest going towards the application of
these bio fertilizers are because of increase capacity of the nutrient uptake of the plants and he increase society of
the society (10,13). Bio fertilizers are mostly used to increases the microbial activity which in return increases the
ABSTRACT
For years chemical fertilizers are used to fulfill the soil requirement of nutrients and yield, but large amount of these chemical fertilizers are
dangerous for environment, beneficial microbes, animals, and humans as well. Therefore, environmental friendly and cost effective biofertilizers are
used. Biofertilizer are the substances which contain microorganisms those microorganisms may be fungi, bacteria, and protozoa which have ability
to increase fertility of soil by Nitrogen fixation, Phosphorous solubilization, and Iron sequestration. These processes convert insoluble form of
nutrients into soluble form and make it available to the roots of plant which easily take them up and utilize them. There are variety of the crops whose
productivity can be increased by applying biofertilizer such as rice, oat, and other grain crops. In this review we go through the way of application of
biofertilizers, and how the help the plants and in which they help.
Keywords: Biofertilizers, PGPR, Auxin, Rhizobium.

availability of the nutrients which can be assimilated easily by the plants (1,14,15) The organic fertilizers are
promoted to get the natural means of getting nutrients by the plants(16) and increase the mobilization (17) which
increase the fertility of the soil and increase the crop yield (18,19).
Why we need bio-fertilizers
General use of chemicals as a fertilizers can full fell the demand of fast growth and high amount of food supply but
obviously those leads to damage the environment (damaging microbial biota, killing friendly insects and increase
in pH). However the use of chemicals fertilizers make the crops susceptible to disease and also results in decease
soil fertility (5,20).
It has been estimated the in 2020 the world population will we more than 8 billon and to feed them we need 28.8
million tons of food and nutrients and we estimated the only 21.6 million tons will be available to community of the
world) (21).
To feed the world population we have to increase the amount of agriculture and increase the productivity in such a
way the it is useful and also show no bad effect on either environment, Human, beneficial insects etc. for this
purpose we have to increase agriculture productions using chemical fertilizers, pesticides, herbicides, fungicides,
and insecticides (22).
As already discussed due to hazardous effect we are moving towards other sources rather the chemicals which have
to be more safe and more effective and ecologically safe and cost effective as well, all these properties are present
in bio-fertilizers (23).
The bio-fertilizers like other man-made chemicals are commercial products having beneficial microbes, substances
important for growth of microbes and other additives which can probably increase the growth of microbes. It was
reported that bio-fertilizer increase the contents of food protein, amino acids, vitamins, and nitrogen and other
essential elements from renege 10%- 40% (24).
The bio-fertilizer provides and help the plant getting both macro and micro essential elements for growth and also
help in supply of hormones and other organic element for plants (25).
The application of bio fertilizer is most effective and natural way to keep the bio system active and working, the
microbes in soil provides nutrients to plants and helping the biodiversity maintains by improving the quality of soil
(26).
How biofertilizers work?
Bacteria, fungi and other microbes an important and unequally distributed micro-organism present everywhere in
the world with different amount of concentration present in more concentration near the root and perform very
important works.
The Bacteria which are present near the roots (rhizosphere) have the capability to promote plant growth
accordingly are called as plant growth promoting microbes, the bacteria which play its role as a plant growth

promoter are called as plant promoting bacteria or (PGPB). Today many of the PGPB are available on commercial
scale as a bio fertilizers (27)
PGPB have very important role in the fertility of soil and plant growth. Its paly role in two ways:
 PGPBs supply important hormones like auxins, cytokinin, gibberellin, which play role in plant growth
directly; PGPBs help in providing the essential elements like nitrogen by nitrogen fixation, phosphorous by
method of phosphorous solubilization, potassium intake, which is called as direct way.
 Helping the genes against the pathogens in many ways which are called as indirect way.(28)
Direct way of action
Auxin
Auxin is a hormone which work in geo and phototropism, tissue differentiation, cell division, and stem root
elongation (29).
PGPBs show positive effect on plant growth as they have the ability to produce the auxin, 80% microbes present in
rhizosphere produce the auxin and their secondary metabolite (30).
Rhizobacteria change the level of auxin concentration in in the form of indole-3-acetic acid (IAA) which can
increase the surface area of roots and large amount of uptake of auxin by plants and remember that going thought
research is has been noted that auxin and IAA are interchangeable (31).
Different cells of different plants are sensitive at different level to auxin (32). Where the PGPB produce the IAA
work as auxin when concentration of IAA is suboptimal roots will be develop and when optimal shoots will be
(28,33).
Ethylene
Ethylene is very important hormone in plant in which at play its role in both cell division and normal growth as well
as also have very significant role in the helping the plant against different types of biotic and abiotic stress (34).
Almost all plant at any stage of their life are effected by ethylene which is influenced by hormones of plant,
temperature, gravity, light, nutrition and various biotic and abiotic stress (28).
High concentration of ethylene help the plant to response to various stresses either temperature, chemicals like
salts, or drought and light, pathogens (35,36).
The PGPB make bond with the roots of plants and take some of the tryptophan released by plant and change it to
IAA which is then released by bacteria and taken by the plant (37).
when the amount of IAA increase it can work both as plant growth regulator and also activate the enzyme of plant
ACC which increase the level of ACC and at last which results in high concentration of ethylene. The PGPBs which
make IAA from the tryptophan of plant can promote the growth of plant as well it can inhibit the growth of plant
though the ethylene production (38).

Cytokinins
Cytokinins are found in algae, bacteria, fungi and higher plants. We didn‟t know much about bacteria producing
cytokinins and their role. Cytokinins are produced in the tip of roots and from there they are transfer to shoot though
xylem. (38)
It control the cell division and differentiation in meristem cells (39)
In many PGPBs the cytokinin genes are evidenced and when they were added to plant surrounding the growth of
the plant increases and as well other Phyto-hormone configuration. (38)
When the bacteria Bacillus subtilis was used as cytokinin producing agent the results shows that the growth of plant
were altered and , when the sinorhizobium melioti over produce cytokinin and which were used as tester for alfalfa
plants against the drought stress. (39)
Gibberellin (GA)
Gibberellin have a group of teracyclic diterpenoid carboxylic acids with C20 or C19 (40)
Gibberellins work in the activation and stimulation of plant growth and other important process like elongation of
stem, seed germination, sitting of fruit, plant flowering (41)
And also inhabit the growth of plant though GAs signaling pathway. (38)
The biochemistry of both plant produce GAs and bacteria GAs are similar and we readily absolved the absence of
GAs as a reduction of side roots number and length. (42)
The pathway though which GA is produce is known in plants and fungi but in bacteria it is still unknown. (38)
Bacteria Bacillus pumilus and Bacillus licheniformis are isolated which have the ability to produce GA. (43)
Nitrogen fixation
Nitrogen is an essential macroelement which is an important element required for plant growth. It is also required
for the formation of nucleic acid, chlorophyll, and proteins. The nitrogen is present in the atmosphere of about
78%.(44,45) This is present in gaseous form so is not available for the plants. Different nitrogen fertilizers made by
industries are available which increase agricultural productivity (38,46,47).
Biological microbial fixation involves the conversion of the nitrogen present in the atmosphere into the form that is
readily uptaken by the plants such as ammonia (46,48).
The conversion of nitrogen into ammonia Need high amount of energy because of the presence of triple bond in the
N2. Many nitrogen-fixing bacteria have been identified which fix the nitrogen symbiotically with the plants such as
legumes. Examples are Rhizobium, sinorhizombium, azorhizombium etc. (7,26). Rhizobial bacteria form colonies
on the root cells and start the root nodule formulation. (38). In this the bacteria act as bacteroid (having no cell wall)
they fix the nitrogen by the help of nitrogenase enzyme and convert it into the ammonia.in this return the plant

provides hem the fixed carbon which shows the mutualism. Different rhizobia species have been used at a
commercial level to give the crop's nitrogen in the form that can they assimilate.(49)
There is side reaction in the working of nitrogenase enzyme in nitrogen cycle that is the reduction of hydrogen ion
into hydrogen. which release into the atmosphere and causing the waste of energy decreasing the working of
nitrogen fixation by 30 % (38).
Few Nitrogen fixation sp. Are
Rhizobium
These are nitrogen-fixing rhizobacteria and belong to the family of Rhizobacea. It includes Rhizobium,
Bradyrhizobium, Sinorhizobium, Azorhizobium, and Mesorhizobium. They have mutualistic association with
leguminous plant roots and those which are free living fix the nitrogen in non-leguminous plant are known as
diazotrophs (50)
It is not a simple process either complex of enzyme are involved in it i.e. Nitrogenase which contains Di
Nitrogenase reductase containing (Fe) iron and molybdenum (Mo) as a cofactor (51).
It convert N2 into to NH3 by using electrons and also oxygen is essential for respiration of Rhizobium sp.(4).
There are three different kinds of Nitrogenase complexes depending upon changes in cofactor of di Nitrogenase
Mo- Nitrogenase, V- Nitrogenase and Fe- Nitrogenase (52).
Some genes are involved in the N2 Fixation which are called „Nf‟genes they are essential for nitrogen fixation in
free-living & nitrogen-fixing organism (53).
The Nif genes are involved in the activation of Fe/Mo cofactor and also for the electron donation, biosynthesis of
cofactors, and functioning and regulatory of enzymes (54).
Experiments show that when plants were inoculated with the strain of Rhizobium wild type strain Retile 68% more
nirtogenase activity was seen, 25-30% increasing in leaf substances and seeds resultant was increase up to 16%.
(55).
Abeles et al 2012 also showed that when rhizobium sp was applied so the ethylene level was also inease which is
one of the major plant regulators and also help in inhibiting of rhizobia infection.
Azospirillum
Azospirillum are gram negative bacteria with ability of nitrogen fixation belong to spirilaceae family (2).
This genus include mant sp like Azospirillum amazonense, Azospirillum halopraeferens, andAzospirillum
brasilense, but among them the most beneficial sp are Azospirillum lipoferum and A. brasilense (21).
They make associated symbiosis with the plants especially with those which have C4 dicarboxylic pathway of
photosynthesis (56).

They produce plant regulators like IAA, GA and cytokinin with work as root development due to which plants are
able to uptake large amount of nutrients (57).
The major advantage of these bacteria is that they full, fell the requirement of nitrogen in paddy, sorghum and
sunflower up to 25%.
Azotobacter
They are the photoautotrophic, aerobic, non-symbiotic free-living bacteria belong to the family azotobacteriaceae,
playing very important role in nitrogen cycle of plant. They are present where soil is alkaline and most commonly
arable soil (58). Azotobacter vinelandii, Azotobacter beijerinckii, Azotobacter insignis,and Azotobacter
macrocytogenes are the common sp of Azotobacor (21). 30 Reported that Different type of hormones i.e.
gibberellins, naphthalene acetic acid is produced by these bacteria which prevent the formation of root pathogen but
enhance the root growth by minerals and nutrients uptake by plant. Vitamins such as vitamin B complex is also
produced by these bacteria.
These type of bacteria are found in different type of crops such as rice, maize, sugarcane, vegetables (Azobactor
play important role in production of some other vitamins such as riboflavin and thiamin (59)
Azolla and Blue-green algae
Azolla contain sp Azolla caroliniana, Azolla microphylla, Azolla filiculoides, and Azolla mexicana (21).
These are present in large amount in paddy field belonging to eight different types of families and enhance the
growth of plant by producing different type of hormones such as Indole acetic acid, Gibberellic acid (21).
To increase the productivity Nitrogen is essential mineral for that and that nitrogen is provided to rice plant by
means of biological nitrogen fixation (4,60).
4-5% of nitrogen is present in Azolla on dry basis and 0.2-0.4% is present on wet basis. Azolla is very important
means of organic fertilizer as it provide nitrogen to rice plant (21).
It also has the ability to decompose the soil fastly and also provide the plant important minerals like phosphorus,
potassium iron, zinc and other micro elements (61).
Phosphate solubilization
The bacteria which solubilize phosphorus are known as phosphate solubilizing bacteria. These bacteria(38,62).
These bacteria give phosphorus to the plants in the form that can be a move to the environment. These bacteria
convert the phosphorus forms present in the environment such as organic and inorganic phosphate into the form
which is available to the plants. These are affected by many conditions i.e. environmental, soil and also the bacterial
strains present there(63,64). The powerful organisms that solubilize the phosphorus are from the genera Bacillus
and Rhizombim(38,65). the mechanism of solubilizing the phosphorus is firstly the utilization of mineral
dissolving compounds(66,67) i.e. oh ions, co2 and other organic compounds the organic compounds then produced
along with the chelates of the oh ions chelate present and cause in the decrease of PH in the release of the

phosphorus. Then the bacteria produced organic acids and then the phosphate solubilizing bacteria excrete it (68).
This process cause the acidification of the microbial cell and its surroundings.
Iron uptake
Iron play different important roles in plants as a photosynthetic system, chlorophyll for light absorbance and take
part in many different biosynthetic mechanisms. the iron present in soil is not sufficient for the plants. Although the
iron is abundant on the earth it cannot be utilized by the plants and microbes due to its presence in insoluble form.
The bacteria convert them into a soluble form by following method. They excrete molecules that bind with iron
called siderophores. These molecules make bind with high affinity to iron. These soluble complexes then taken by
bacteria or plants and convert it into ferrous or iron. Siderophores have low molecular weight having iron-binding
groups.(38,69,70)
Indirect methods
Many plant diseases are causing the loss of yield of many plants of about 10% in many developed countries (38).
The scientists are promoting the PGPB in case of decrease of the chemicals.
Antibiotics
The PGPBs control the effect of many pathogen attacks by producing antibiotics that will act against the pathogen
and prevent the plant from the disease. The PGPBs produce different antibiotic at different conditions. we can alter
the PGPBs producing antibiotics in laboratories conditions.(71)
Cell wall degrading enzymes
Some plants produce cell wall degrading enzymes which act against fungus. for example, chitinase produced by the
plants used to degrade the chitin of the fungus. The PGPBs also producing many cell wall degrading enzymes when
they were studying in the lab to become an active biocontrol agent. So the PGPBs give protection to the plants by
degrading the cell wall of the fungus.(43)
Hydrogen cyanide
Many PGPBs produce hydrogen cyanide. The hydrogen cyanide is used as a biocontrol agent against many
diseases. The low level of the hydrogen cyanide does not allow the fungus pathogens to attack the plants. So, the
PGPBs show effective resistance against the disease by producing the hydrogen cyanide. the hydrogen cyanide
works by inhibiting the cytochrome c oxidase and its other metabolites. Many bacteria also produce HCN as
Rhizobium, Pseudomonas, and Bacillus.(38,72)
Application of Biofertilizer
Increase in the growth of plants and crops
Many applications of microbial fertilizers motivated the scientists to essay for microbes which show plant growth
enhancing capabilities by increasing the nutrient standard of plants directly or indirectly (2).Some PGPR provoke
the growth by representing their actions as biofertilizers. Plant growth promoting bacteria play important role in

producing growth-regulating hormones. They improve the roots of plant and increase capacity of roots to take
nutrients (73). For the past few years, large number of investigators have learned that PGPR has the ability to
enhance crop growth and to control the plant invaders even under the greenhouse condition (38,68). Biofertilizers
significantly enhance the plant length, number of branches, root and shoot length and also collection of dry matter
into the organs of plants. PGPR vary in their mechanism of action of plant growth usually influence on growth of
plant through solubilization of inorganic phosphate and the production of plant growth regulators.B.megaterium the
plant growth promoting bacteria enhances the growth of root and development for that reason better use of nutrient
will take place (69). Biofertilizers colonize the rhizosphere of plant and enhance the growth signals to the target
crop. The Azospirillum species are known to increase the output of forage and cereal grasses by increasing the
development of roots. Azospirillum proved beneficial for the plant by enhancing the nitrogen-fixing activity of
bacteria in rhizosphere of plants. It helps in the development of root and shoot (74)
Increasing crop productivity
Biofertilizers can be used as an economic input to enhance the productivity of crop; doses of fertilizers could be
reduced and nutrients in high quality from soil can be harvested. The technology of production in case of biological
fertilizers is simple and cost-effective in comparison to synthetic fertilizer plants.
When biofertilizers are employed as a soil inoculants they proliferate and play a role in ecological cycle of nutrients
and proved helpful for the productivity of crops (75).
Growth and yield of plant have been upgraded by frequent inoculation of very potent rhizobacteria (26) and
inoculation with(1).
It is reported that microorganisms such as phosphate solubilizing bacteria which we use as biofertilizer can increase
the yield up to 200-500kg/ha and therefore superphosphate of about 30-50kg can be stored.
We conducted experiments on Bray-1P and cabbage by applying chemical fertilizers and organic biofertilizers to
evaluate their effects on the yield of cabbage and bray-1P.
Role of biofertilizer in photosynthesis
Higher photosynthesis show improve growth rate of plant as about 90% of plant body is achieved through carbon
dioxide assimilation by photosynthesis (76). It was announced that some strains of Rhizobia sufficiently increase
the plant leaves surface area, plant rate of photosynthesis, stomatal opening and closing & efficiency of water
availability showing that the photosynthetic ability of plant can be increased by Rhizobial administration (77).
Leaves are major part of plant having photosynthetic organs (chlorophyll). Therefore number of leaves are very
important. Increase in number of leaves may activate the high growth of roots and also improve the transportation
of water taken by plant roots and also improve the accumulation of minerals and yield (38)
Cost Effective
Biofertilizers on action increase the soil fertility without leaving any harmful effect in the soil and they are much
more cost effective.

There is a need for that agriculture system which is sustainable, in which the fertility of soil is maintained by using
cost-effective resources which should be renewable such as biological fertilizers.
The need for decreasing the costs of fertilizing crops with reconvert able energy forms has revitalized the
importance of biofertilizers all around the world.
Chicken manure as a biofertilizer containing a higher nitrogen content in comparison to expensive inorganic or
synthetic fertilizers, easily available, hence it is easily affordable for the farmers. (2)
Mode of action of bio-fertilizers
Bio fertilizers can be applied in many ways into the soil
 Dry biofertilizers are heterogeneous with the seeds
 Biofertilizers applied in powdered form in soil.
 A little amount of water is added into the seed hopper and then biofertilizers are add this is called ass
sprinkle method.
 Biofertilizers are used in suspended form in water after that added to seeds this is called slurry method.
 Method of seed pelleting (coating the seed with biofertilizer)
 In a slurry the seed and biofertilizers are mixed and adhesive are applied and coat the seed with lime like
materials.
 During the sowing the peat suspension in water sprayed into the furrow.
 Inoculation seed treatment.
 Apply in soil
 Apply in seed links or root dip.(78)
Future perspective
For the decades the development and growth of the plants were depended on the conventional methods like
chemical fertilizers (62,79,80)
Which increase the production of yield up to a limit but they also make the environment pollute and the use of
chemicals were hazardous to both human and beneficial microbes.
A review clearly brings us to the point that bio fertilizers are the sources of increasing the growth as well as
nutritional amount in crops. Different types of bio fertilizer are in practice and the field of bio fertilizer is an
emerging one in which many microbes are used to increase the yield of crop as well as protect it from different
types of pathogens. The use of bio fertilizers are very useful and it believe that it will growth with time (81)
But in future few issues regarding bio fertilizers will need to be addressed and serious action have to be taken to
solve those (28).

And also require research on the performance of different efficient stains and also their effect on other beneficial
microbes and if the conditions change what will be there effect and performance.
Conclusion
At last of our review we concluded that today microbial based fertilizers are very significant for the improvements
crop. There are two means due to which we have to use the bio fertilizers. First one that they provide the
unavoidable amount of yield and nutrition to human food, they are very safe to use for environment, plants and
animals and human and highly eco-friendly. Second one is that they ensure the sustainable growth of agriculture by
providing the nutrition to plant in it rhizosphere such as N, P, and K and other minerals and vitamins. Different
types microbes are used for this purpose like PGPB and fungi which work exceptional well.
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