This document discusses sanitation, disinfection, and fumigation procedures in hatcheries. It emphasizes the importance of regular cleaning and disinfection to control microbe multiplication. Thoroughly cleaning removes up to 85% of microbes. Disinfectants should be effective, safe for equipment, and compatible with water. Fumigation with potassium permanganate and formalin is an effective way to disinfect setter trays, hatchers, and eggs. Hatchery waste should also be properly disposed of to prevent disease transmission. Microbiological monitoring through bacterial and fungal cultures helps evaluate the effectiveness of the total sanitation program.