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Prepared by
Dr Muhammad Ashiq Toor
DVM , B s c (Zoology)
Islamia University of Bahawal Pur (IUB) (2010 – 2015)
+92-344-499-7375
+92-300-364-2402
Sanitation Disinfection and fumigation in Hatchery
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Sanitation Disinfection and
fumigation in Hatchery
Sanitation
The process of keeping places free from
ØDirt
ØInfection
ØDisease
ØRemoving wastes
The hatcher is
the main source
of
contamination
for the entire
hatchery.
Debris, dead chicks, and chick fluff are
ideal replication sites for bacteria and
fungi
Øby cleaning
Cleaning and Disinfection
Completely free of Microbs
Impossible
Regular cleaning and
disinfection control microbs
multiplication
Remove organic materials
A good cleaning removes up
to 85% of microbs
Cleaning & Disinfection
Good design
for
Cleaning
Disinfection
Effective
Disease
control in
Hatchery
Cleaning Sanitation is always 90% Cleaning
Empty refuse
Soak in
detergents
Wash with hot
water
Rinse
Disinfect
Mach C Solutions
Netherland
Disinfectants and detergents for
Equipment
Effective disinfectant
property
Safe for Equipment
Compatible with water
ØSanitizer reduces to “safe levels”
ØDisinfectant kills 100% bacteria
selection
Walls, Floors, Ceilings,
Windows, Fans, Ducting, and
Machines Remove dust and debris
 Where necessary, scrub walls and
floors
 Switch on setter machine to warm up
 Broad-spectrum disinfectant detergent
solution to the setter machine
 Use pressure washing and leave them
for drying


Cleaning
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
USA
Canada
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Fumigation ( KmNO4 + Formalin) 1:2
Formaldehyde is effective biocide
Reacting with carboxyl, amino,
hydroxyl and sulfhydryl groups of
proteins
Effective at room Temp and RH 70%
Procedure
Fumigation ( KmNO4 + Formalin (3x)
• Sett the eggs in
setter trays
Move them to
fumigation room
• KmNO4 60gram
• Formalin 120ml
• That is 3X concentration
Per 100 cubic
feet
• Circulate for 20 minutes
• Expel gas after that by exhaust fan
• At room tem.
Fumigation of eggs in setters (1X)

Eggs should be fumigated
within 12 h after setting
Eggs which have been
incubated for 24-96 h
should not be fumigated
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Fumigation of hatchers (1x)
•Removal of all chicks
•The cleaning and disinfection of the empty
machine.
•The disinfected egg trays are replaced
•The machine is prepared for the next
batch of incubating eggs.
•The doors and vents should be closed
•Fumigation time should be at least three
hours, or preferably overnight
Procedure
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Neutralisation of formaldehyde gas
Formaldehyde gas may be neutralised in 10-
15 min using ammonium hydroxide at an
amount equal to half the volume of formalin
used
Egg Washing
Easier , Faster
Clean residuals on eggs
Avoid contamination to
other eggs by residuals
Some managers prefer washing on fumigation
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Egg Washing effectively sanitize the
hatching eggs if
Egg Washing
Proper equipment's
Proper temperature of solution
Washing material, detergents
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor
Disinfectants and detergents for
egg washing
Quaternary Ammonium
ROCCAL-D®
10% Quaternary
Ammonium
disinfectant
4.2 %
Sodium
Carbonate
0.4 %
EDTA
Egg Washing
Chlorine based disinfectants
widely used for table eggs and
also safe for hatching eggs
Quaternary Ammonium an
excellent sanitizer
Hatchery Waste Disposal
Infertile
eggs
Non-
hatched
eggs
Dead
weak or
unsalable
chicks
Egg
shells
These wastes should be properly disposed of, otherwise they can be a source
of disease infection in the hatchery.
Evaluating your total Sanitation
program

Hatchability
percentages
Embryo
mortality
rates
Number
(percentage) of
rotten eggs in
setter
First 4 day
post hatching
mortality rates
of chicks
Results from
Bacterial and
Fungal
cultures
MICROBIOLOGICAL MONITORING
OF HATCHERIES

Simplest way is to use agar plates.
For Bacterial cultures - put them in zip lock
bags and put them in Setters for 2 days.
For Fungal cultures put them in zip lock bags
and put them out at room temp for 4-5 days.
Procedure in the hatchery
Put them out in different parts of hatchery
building (label them) for 10 minutes.
The test in the hatchery is performed
routinely once a month
Preparation of media
Agar sausages Agar broth
Evaluation of results
Setter & Egg Rooms (after cleaning):
Number of colonies per air plate
Bacteria Mold Rating
0-10 0 Clean
11-20 1-3 Slightly contaminated
21-30 4-6 Moderately contaminated
31 or
more
7 or
more
Heavily contaminated
After incubation of samples at
37°C for 16-20 h, the number of
bacterial colonies growing on
each sample
Setter & Egg Rooms (after cleaning):
Number of colonies per air plate
Heavily contaminated7 or
more
31 or
more
Moderately contaminated4-621-30
Slightly contaminated1-311-20
Clean00-10
RatingMoldBacteria
Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor

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Sanitation disinfection and fumigation in hatchery by Dr M. Ashiq Toor

  • 1. Prepared by Dr Muhammad Ashiq Toor DVM , B s c (Zoology) Islamia University of Bahawal Pur (IUB) (2010 – 2015) +92-344-499-7375 +92-300-364-2402 Sanitation Disinfection and fumigation in Hatchery
  • 4. Sanitation The process of keeping places free from ØDirt ØInfection ØDisease ØRemoving wastes The hatcher is the main source of contamination for the entire hatchery. Debris, dead chicks, and chick fluff are ideal replication sites for bacteria and fungi Øby cleaning
  • 5. Cleaning and Disinfection Completely free of Microbs Impossible Regular cleaning and disinfection control microbs multiplication Remove organic materials A good cleaning removes up to 85% of microbs
  • 6. Cleaning & Disinfection Good design for Cleaning Disinfection Effective Disease control in Hatchery
  • 7. Cleaning Sanitation is always 90% Cleaning Empty refuse Soak in detergents Wash with hot water Rinse Disinfect Mach C Solutions Netherland
  • 8. Disinfectants and detergents for Equipment Effective disinfectant property Safe for Equipment Compatible with water ØSanitizer reduces to “safe levels” ØDisinfectant kills 100% bacteria selection
  • 9. Walls, Floors, Ceilings, Windows, Fans, Ducting, and Machines Remove dust and debris  Where necessary, scrub walls and floors  Switch on setter machine to warm up  Broad-spectrum disinfectant detergent solution to the setter machine  Use pressure washing and leave them for drying   Cleaning
  • 11. USA
  • 15. Fumigation ( KmNO4 + Formalin) 1:2 Formaldehyde is effective biocide Reacting with carboxyl, amino, hydroxyl and sulfhydryl groups of proteins Effective at room Temp and RH 70%
  • 16. Procedure Fumigation ( KmNO4 + Formalin (3x) • Sett the eggs in setter trays Move them to fumigation room • KmNO4 60gram • Formalin 120ml • That is 3X concentration Per 100 cubic feet • Circulate for 20 minutes • Expel gas after that by exhaust fan • At room tem.
  • 17. Fumigation of eggs in setters (1X)  Eggs should be fumigated within 12 h after setting Eggs which have been incubated for 24-96 h should not be fumigated
  • 19. Fumigation of hatchers (1x) •Removal of all chicks •The cleaning and disinfection of the empty machine. •The disinfected egg trays are replaced •The machine is prepared for the next batch of incubating eggs. •The doors and vents should be closed •Fumigation time should be at least three hours, or preferably overnight Procedure
  • 21. Neutralisation of formaldehyde gas Formaldehyde gas may be neutralised in 10- 15 min using ammonium hydroxide at an amount equal to half the volume of formalin used
  • 22. Egg Washing Easier , Faster Clean residuals on eggs Avoid contamination to other eggs by residuals Some managers prefer washing on fumigation
  • 24. Egg Washing effectively sanitize the hatching eggs if Egg Washing Proper equipment's Proper temperature of solution Washing material, detergents
  • 27. Disinfectants and detergents for egg washing Quaternary Ammonium ROCCAL-D® 10% Quaternary Ammonium disinfectant 4.2 % Sodium Carbonate 0.4 % EDTA Egg Washing Chlorine based disinfectants widely used for table eggs and also safe for hatching eggs Quaternary Ammonium an excellent sanitizer
  • 28. Hatchery Waste Disposal Infertile eggs Non- hatched eggs Dead weak or unsalable chicks Egg shells These wastes should be properly disposed of, otherwise they can be a source of disease infection in the hatchery.
  • 29. Evaluating your total Sanitation program  Hatchability percentages Embryo mortality rates Number (percentage) of rotten eggs in setter First 4 day post hatching mortality rates of chicks Results from Bacterial and Fungal cultures
  • 30. MICROBIOLOGICAL MONITORING OF HATCHERIES  Simplest way is to use agar plates. For Bacterial cultures - put them in zip lock bags and put them in Setters for 2 days. For Fungal cultures put them in zip lock bags and put them out at room temp for 4-5 days. Procedure in the hatchery Put them out in different parts of hatchery building (label them) for 10 minutes. The test in the hatchery is performed routinely once a month Preparation of media Agar sausages Agar broth
  • 31. Evaluation of results Setter & Egg Rooms (after cleaning): Number of colonies per air plate Bacteria Mold Rating 0-10 0 Clean 11-20 1-3 Slightly contaminated 21-30 4-6 Moderately contaminated 31 or more 7 or more Heavily contaminated After incubation of samples at 37°C for 16-20 h, the number of bacterial colonies growing on each sample Setter & Egg Rooms (after cleaning): Number of colonies per air plate Heavily contaminated7 or more 31 or more Moderately contaminated4-621-30 Slightly contaminated1-311-20 Clean00-10 RatingMoldBacteria

Editor's Notes

  • #28: Quaternary Ammonium : alkyldimethyl benzyl ammonium chloride
  • #31: ‘1.Agar sausages' are prepared by adding 35 g agar powder, 2.5 g 'meat infusion broth' and 8 g sodium thiosulfate granules (or 5 g sodium thiosulfate powder) to 11 of sterile water. 2.(e.g. floors, walls, tables, trolleys, egg trays, tops and interior of incubators, egg and chicken boxes, sexing