HYBRID SEED PRODUCTION
TECHNOLOGY IN SORGHUM
1
2
Dr.J.I.Nanavati
NAME
MANAV SOLANKI
CHIRAG VASAVA
Reg. No.
4011120036
4011120042
PREPARED BY:
SUBMITTED TO:
ASSISTANT PROFESSOR
S.M.C.P.A, Anand
CONTENT
 Botanical Information
 Economic Importance
 Floral Biology
 Area Under Sorghum Production
 Life Cycle
 Breeding Objective
 Season
 Soil Requirement
 Field Preparation
3
TO BE CONTINUE...
 Fertilizer Application
 Sowing Method
 Procedure For Hybrid Seed Production
 Isolation Distance
 Planting Ratio
 Spacing
 Seed Rate
 Synchronization Technique
 Roughing From Female Plot
4
TO BE CONTINUE...
 Irrigation
 Fertilizer Application
 Weeding
 Plant Protection
 Stages of Seed Production
 Number of Inspections
 Method of Harvesting
 Threshing
 Drying
5
TO BE CONTINUE...
 Processing
 Seed Treatment (For Storage)
 Packing & Storage
 Some Important Hybrid Varieties
 Seed Standard
 Research Station
6
BOTANICAL INFORMATION
Name of Crop : Sorghum
Botanical Name : Sorghum bicolor L.
Family : Poaceae
Chromosome Number : 2n = 20
Center of origin : North East of Africa
Mode of Pollination : Often Cross Pollinated
Out Cross Percentage : 6-45 %
7
ECONOMIC IMPORTANCE
 Sorghum is one of the main staple
food crops for the world’s poor and
food insecure people in the semi-arid
tropics of Africa and Asia.
 It is an important source of feed,
fodder and bio-fuel apart from food.
 It is known to be rich in phenolic
compounds, many of which act as
antioxidants.
8
TO BE CONTINUE...
 Sorghum grain contains 11.3% protein,
3.3% fat and 56-73% starch.
 It is relatively rich in iron,
zinc,phosphorus and b-complex
vitamins
 Tannins, found particularly in red
grained types, contain antioxidants
that protect against cell damage, a
major cause of diseases and aging.
9
TO BE CONTINUE...
 Sorghum starch is gluten-free, making
sorghum a good alternative to wheat
flour for individuals suffering from
celiac disease.
10
FLORAL BIOLOGY
 Sorghum is an often cross pollinated
crop. The extent of out crossing is 6-45%
and depends on nature of ear head.
 In loose panicles the cross pollination is
more and less in compact panicle.
 Spikelets occur in pairs on the lateral
branches of the panicle. One is sessile
while the other spikelet is pedicelled.
 Sessile is bisexual and pedicelled spikelet
and each spikelet has two florets.
11
TO BE CONTINUE...
 Flower opening starts after 2 to 4 days
of emergence of panicle from the boot
leaf.
 Fertile pollen will be lemon yellow in
colour.
 Receptivity of stigma starts two days
before opening and remains for
several days(5 days).
 Flower opening and anthesis will be
from 2 am to 8 am.
12
TO BE CONTINUE...
 Sorghum flower:-
13
AREA UNDER PRODUCTION
 For 2022-23, USDA has projected
world sorghum area as 41.04 million
hectares and production as 60.38
million tonnes.
 For India, the same was projected as
4.50 million hectares and 4.40 million
tonnes.
14
LIFE CYCLE
15
BREEDING OBJECTIVES
 High yield
 Better quality
 Early maturity
 Wider adaptation
 Resistance to biotic and abiotic
stresses
 Low HCN content
 Leafy & Sweet
16
For Fodder
SEASON
 The best season for production is
November-December and the
flowering should not coincide either
with rain or high RH as it will wash
out the pollen and the maturation
should coincide with dry weather.
 The temperature of 37oC is favourable
for better seed setting.
17
SOIL REQUIREMENT
 Sorghum can be grown successfully on
clay, clay loam or sandy loam soil.
 Fertile, well drained soils are important
to optimize yield.
 Sorghum is generally grown in almost
neutral(pH 6.5 to 7.0) to moderately
alkaline(pH 7.0 to 8.3) soils.
 Optimum soil pH is 6.5 to 7.8;
development and yield are hampered in
soils with pH below 5.7 or above 8.3.
18
FIELD PREPARATION
 Plough the field with an iron plough
and twice with a country plough. Fine
tilth is not needed as it affects
germination and yield.
 In soils with sub-soil hard pan,
chiselling should be done every year at
the start of each season. After
chiselling, plough once with disc
plough and twice with cultivator.
19
FERTILIZER APPLICATION
 Compost : 12.5 t/ha
20
N P K
Basal 100 50 50
Top dressing 50 0 0
Total 150 50 50
SOWING METHOD
 Seeds are
sown by
drilling
method.
 In some
places seeds
are also
raised in
nursery and
transplanted
to the main
field
21
PROCEDURE FOR HYBRID
SEED PRODUCTION
 In sorghum hybrid seed is produced by
utilizing
cytoplasmic genetic male sterile system.
 The 1st source of male sterile cytoplasm used
is Combined kafir 60 A.
 Hybrid seed production involves two steps;
1. Maintenance of parental Lines (A-line, B-
line and R-line)
2. Commercial hybrid seed production (AxR)
22
TO BE COUNTINUE...
 Maintenance of parental lines is generally
referred as foundation seed production and
hybrid seed production as certified seed class.
 The A-line can be maintained by crossing
with B-line in an isolated plot, while in hybrid
seed production A-line is crosses with R-line
or fertility restorer line.
 The B-line and the R-line can be maintained
just like normal varieties by following the
required isolation and field standards.
23
Maintenance of A-line or Hybrid
seed Production (AxR):
Land requirement:
 Land should be free from volunteer
plants, Johnson grass, Sudan grass and
other forage types.
 The same crop should not be grown on
the same piece of land in the previous
one season unless it is the same variety
and certified by certification agency for
its purity
24
ISOLATION DISTANCE
Isolation distance(m)
Foundation Seed Certified Seed
Normal 200 100
On presence of Johnson grass 400 400
On presence of forage sorghum 400 200
Hybrids 300 200
25
PLANTING RATIO
 For Foundation Seed :- 4:2 (A:B)
 For Certified Seed :- 5:2 (A:R)
26
SPACING
 A Line : 45cm x 30cm
 R Line : 45cm x Solid row spacing
27
SEED RATE
 A Line : 8 kg/ha
 R Line : 4 kg/ha
28
SYNCHRONIZATION
TECHNIQUE
 Proper synchronization of flowering
between A-Line and R-Line is a common
problem.
 If the difference between the male and
female parent is less than a week, it can
be manipulated by cultural practices.
 Spraying growth retardant MH 500ppm
at 45 DAS, delays flowering in advancing
parent.
 Withhold one irrigation to the advancing
parent.
 Urea Spraying 1% to the lagging parent.
29
ROUGHING
 DO IT IN BOTH PARENTS.
Off types :-
• In female line : Wild types, Pollen
shedders, R-Line.
• In male line : A-Line, Diseased plants,
wild types.
30
IRRIGATION
 The crop should be irrigated once in a
week for enhanced seed set and
formation of bolder grains.
 The critical stages of irrigation are
primordial initiation stage, vegetative
stage, milky and maturation stage.
 If the irrigation is withheld in these
stages seed set will be poor and seed size
will be reduced.
31
WEEDING
 Application of atrazine @ 10ml per
litre as pre-emergence herbicide
control the growth of weeds upto 20-
25 days.
 One hand weeding at the time of
primordial initiation keep the field
free of weeds.
32
PLANT PROTECTION
PEST:-
33
Common pests Management techniques
Shootfly Monocrotophos 0.03%
Stemborer Rogar 0.3%
Gall midge Endosulphan 0.07%
Earhead bugs Endosulphan 0.07%
Black mould & sugary disease Endosulphan 0.07%+ Bavistin
@10g/lit
Kernal smut & head smut Endosulphan 0.07%+ Bavistin
@10g/lit
STAGES OF SEED
PRODUCTION
 Breeder seed A x B - B - R
 Foundation seed A x B - B - R
 Certified seed A x R
34
NO. OF INSPECTION
A minimum of three inspections shall be made
as follows.
1. The first inspection shall be made before
flowering on order to verify isolation,
volunteer plants, and other relevant factors.
2. The second inspection shall be made during
flowering to check isolation, off types and
other relevant factors.
3. The third inspection shall be made at
maturity and prior to harvesting to verify
true nature of plant and other relevant
factors.
35
METHOD OF HARVESTING
 The crop attains physiological maturity
40-45 days after 50% flowering and the
seed moisture at this stage will be around
25-30%.
 This stage can be easily identified by the
formation of dunken layer at the place of
attachment to the ear head.
 The earheads are harvested
commercially when 80% of the earheads
are physiologically matured,where the
moisture content will be around 20%.
36
THRESHING
 The earheads are dried under sun and
threshed with stick for extraction of
seeds.
 The moisture content of seed at the time
of threshing will be 15-18%.
 On large scale production LCT threshers
are used, but care should be given to
avoid mechanical damage, which in turn
will reduce the seed quality and
storability.
SEED YIELD :-
 3000-4000 kg/ha
37
DRYING
 The seeds are dried to 8-10% moisture
content either under sun or adopting
mechanical driers for long term
storage as the seeds is orthodox in
nature.
38
PROCESSING
 Mechanical grading can be done with
cleaner cum grader, which will remove
the undesired immature and chaffy
seeds.
 The middle screen size should be
9/64’’ round perforated sieves. The
size can vary depending on the type of
seed.
 For fodder sorghum 8/64’’ sieve is
used.
39
SEED TREATMENT (FOR
STORAGE)
 The seeds are infested with several
storage pests, to protect against these
pests the seeds are given protective
treatment with bavistin @2g/kg of
seed with carbaryl @200mg/kg of
seed as slurry treatment.
 Bifenthrin @5mg/kg of seed is also
recommended for fodder sorghum.
40
SEED PACKING & STORAGE
 Seeds are packed in gunny bags for short
term storage while in HDPE and polylined
gunny bag for long term storage.
 The treated seed can be stored up to 12
months provided the seeds are not infected
with storage pests.
 Seed can be stored up to 3 years if the seeds
are packed in moisture containers and are
stored at low temperature.
 The godown should be kept clean as the
possibility of secondary infestation with
Trifolium(red flour weevil) is much in these
crop.
41
SOME IMPORTANT HYBRID
VARIETIES
HYBRID PARENTS
CSH1 CK 60 A x IS 84
CSH5 2077A x CS3541
CSH9 MS 296 x CS 3541
COH2 2219A x IS3541(Kovilpatti Tall)
COH3 2077A x CO21
COH4 296A x TNS30
CSH 13 R 296 A x RS 29
CSH14 AKMS 14A x AKR 150
CSH16 27 A x C43
CSH15 (R) 104 A x R 585
CSH17 AKMS 14A x RS 673
42
SEED STANDARD
FOUNDATION
SEED
CERTIFIED SEED
Physical Purity(%) 98 98
Inert matter(%) 2 2
Other crop seed 5/kg 5/kg
Weed seed 10/kg 20/kg
Other distinguishable
variety
10/kg 20/kg
Ergot disease by
number
0.020% 0.040%
Moisture content
Moisture previous
container
12 12
Moisture vapour proof
container
8 8
43
44

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SORGHUM-2.pptx

  • 2. 2 Dr.J.I.Nanavati NAME MANAV SOLANKI CHIRAG VASAVA Reg. No. 4011120036 4011120042 PREPARED BY: SUBMITTED TO: ASSISTANT PROFESSOR S.M.C.P.A, Anand
  • 3. CONTENT  Botanical Information  Economic Importance  Floral Biology  Area Under Sorghum Production  Life Cycle  Breeding Objective  Season  Soil Requirement  Field Preparation 3
  • 4. TO BE CONTINUE...  Fertilizer Application  Sowing Method  Procedure For Hybrid Seed Production  Isolation Distance  Planting Ratio  Spacing  Seed Rate  Synchronization Technique  Roughing From Female Plot 4
  • 5. TO BE CONTINUE...  Irrigation  Fertilizer Application  Weeding  Plant Protection  Stages of Seed Production  Number of Inspections  Method of Harvesting  Threshing  Drying 5
  • 6. TO BE CONTINUE...  Processing  Seed Treatment (For Storage)  Packing & Storage  Some Important Hybrid Varieties  Seed Standard  Research Station 6
  • 7. BOTANICAL INFORMATION Name of Crop : Sorghum Botanical Name : Sorghum bicolor L. Family : Poaceae Chromosome Number : 2n = 20 Center of origin : North East of Africa Mode of Pollination : Often Cross Pollinated Out Cross Percentage : 6-45 % 7
  • 8. ECONOMIC IMPORTANCE  Sorghum is one of the main staple food crops for the world’s poor and food insecure people in the semi-arid tropics of Africa and Asia.  It is an important source of feed, fodder and bio-fuel apart from food.  It is known to be rich in phenolic compounds, many of which act as antioxidants. 8
  • 9. TO BE CONTINUE...  Sorghum grain contains 11.3% protein, 3.3% fat and 56-73% starch.  It is relatively rich in iron, zinc,phosphorus and b-complex vitamins  Tannins, found particularly in red grained types, contain antioxidants that protect against cell damage, a major cause of diseases and aging. 9
  • 10. TO BE CONTINUE...  Sorghum starch is gluten-free, making sorghum a good alternative to wheat flour for individuals suffering from celiac disease. 10
  • 11. FLORAL BIOLOGY  Sorghum is an often cross pollinated crop. The extent of out crossing is 6-45% and depends on nature of ear head.  In loose panicles the cross pollination is more and less in compact panicle.  Spikelets occur in pairs on the lateral branches of the panicle. One is sessile while the other spikelet is pedicelled.  Sessile is bisexual and pedicelled spikelet and each spikelet has two florets. 11
  • 12. TO BE CONTINUE...  Flower opening starts after 2 to 4 days of emergence of panicle from the boot leaf.  Fertile pollen will be lemon yellow in colour.  Receptivity of stigma starts two days before opening and remains for several days(5 days).  Flower opening and anthesis will be from 2 am to 8 am. 12
  • 13. TO BE CONTINUE...  Sorghum flower:- 13
  • 14. AREA UNDER PRODUCTION  For 2022-23, USDA has projected world sorghum area as 41.04 million hectares and production as 60.38 million tonnes.  For India, the same was projected as 4.50 million hectares and 4.40 million tonnes. 14
  • 16. BREEDING OBJECTIVES  High yield  Better quality  Early maturity  Wider adaptation  Resistance to biotic and abiotic stresses  Low HCN content  Leafy & Sweet 16 For Fodder
  • 17. SEASON  The best season for production is November-December and the flowering should not coincide either with rain or high RH as it will wash out the pollen and the maturation should coincide with dry weather.  The temperature of 37oC is favourable for better seed setting. 17
  • 18. SOIL REQUIREMENT  Sorghum can be grown successfully on clay, clay loam or sandy loam soil.  Fertile, well drained soils are important to optimize yield.  Sorghum is generally grown in almost neutral(pH 6.5 to 7.0) to moderately alkaline(pH 7.0 to 8.3) soils.  Optimum soil pH is 6.5 to 7.8; development and yield are hampered in soils with pH below 5.7 or above 8.3. 18
  • 19. FIELD PREPARATION  Plough the field with an iron plough and twice with a country plough. Fine tilth is not needed as it affects germination and yield.  In soils with sub-soil hard pan, chiselling should be done every year at the start of each season. After chiselling, plough once with disc plough and twice with cultivator. 19
  • 20. FERTILIZER APPLICATION  Compost : 12.5 t/ha 20 N P K Basal 100 50 50 Top dressing 50 0 0 Total 150 50 50
  • 21. SOWING METHOD  Seeds are sown by drilling method.  In some places seeds are also raised in nursery and transplanted to the main field 21
  • 22. PROCEDURE FOR HYBRID SEED PRODUCTION  In sorghum hybrid seed is produced by utilizing cytoplasmic genetic male sterile system.  The 1st source of male sterile cytoplasm used is Combined kafir 60 A.  Hybrid seed production involves two steps; 1. Maintenance of parental Lines (A-line, B- line and R-line) 2. Commercial hybrid seed production (AxR) 22
  • 23. TO BE COUNTINUE...  Maintenance of parental lines is generally referred as foundation seed production and hybrid seed production as certified seed class.  The A-line can be maintained by crossing with B-line in an isolated plot, while in hybrid seed production A-line is crosses with R-line or fertility restorer line.  The B-line and the R-line can be maintained just like normal varieties by following the required isolation and field standards. 23
  • 24. Maintenance of A-line or Hybrid seed Production (AxR): Land requirement:  Land should be free from volunteer plants, Johnson grass, Sudan grass and other forage types.  The same crop should not be grown on the same piece of land in the previous one season unless it is the same variety and certified by certification agency for its purity 24
  • 25. ISOLATION DISTANCE Isolation distance(m) Foundation Seed Certified Seed Normal 200 100 On presence of Johnson grass 400 400 On presence of forage sorghum 400 200 Hybrids 300 200 25
  • 26. PLANTING RATIO  For Foundation Seed :- 4:2 (A:B)  For Certified Seed :- 5:2 (A:R) 26
  • 27. SPACING  A Line : 45cm x 30cm  R Line : 45cm x Solid row spacing 27
  • 28. SEED RATE  A Line : 8 kg/ha  R Line : 4 kg/ha 28
  • 29. SYNCHRONIZATION TECHNIQUE  Proper synchronization of flowering between A-Line and R-Line is a common problem.  If the difference between the male and female parent is less than a week, it can be manipulated by cultural practices.  Spraying growth retardant MH 500ppm at 45 DAS, delays flowering in advancing parent.  Withhold one irrigation to the advancing parent.  Urea Spraying 1% to the lagging parent. 29
  • 30. ROUGHING  DO IT IN BOTH PARENTS. Off types :- • In female line : Wild types, Pollen shedders, R-Line. • In male line : A-Line, Diseased plants, wild types. 30
  • 31. IRRIGATION  The crop should be irrigated once in a week for enhanced seed set and formation of bolder grains.  The critical stages of irrigation are primordial initiation stage, vegetative stage, milky and maturation stage.  If the irrigation is withheld in these stages seed set will be poor and seed size will be reduced. 31
  • 32. WEEDING  Application of atrazine @ 10ml per litre as pre-emergence herbicide control the growth of weeds upto 20- 25 days.  One hand weeding at the time of primordial initiation keep the field free of weeds. 32
  • 33. PLANT PROTECTION PEST:- 33 Common pests Management techniques Shootfly Monocrotophos 0.03% Stemborer Rogar 0.3% Gall midge Endosulphan 0.07% Earhead bugs Endosulphan 0.07% Black mould & sugary disease Endosulphan 0.07%+ Bavistin @10g/lit Kernal smut & head smut Endosulphan 0.07%+ Bavistin @10g/lit
  • 34. STAGES OF SEED PRODUCTION  Breeder seed A x B - B - R  Foundation seed A x B - B - R  Certified seed A x R 34
  • 35. NO. OF INSPECTION A minimum of three inspections shall be made as follows. 1. The first inspection shall be made before flowering on order to verify isolation, volunteer plants, and other relevant factors. 2. The second inspection shall be made during flowering to check isolation, off types and other relevant factors. 3. The third inspection shall be made at maturity and prior to harvesting to verify true nature of plant and other relevant factors. 35
  • 36. METHOD OF HARVESTING  The crop attains physiological maturity 40-45 days after 50% flowering and the seed moisture at this stage will be around 25-30%.  This stage can be easily identified by the formation of dunken layer at the place of attachment to the ear head.  The earheads are harvested commercially when 80% of the earheads are physiologically matured,where the moisture content will be around 20%. 36
  • 37. THRESHING  The earheads are dried under sun and threshed with stick for extraction of seeds.  The moisture content of seed at the time of threshing will be 15-18%.  On large scale production LCT threshers are used, but care should be given to avoid mechanical damage, which in turn will reduce the seed quality and storability. SEED YIELD :-  3000-4000 kg/ha 37
  • 38. DRYING  The seeds are dried to 8-10% moisture content either under sun or adopting mechanical driers for long term storage as the seeds is orthodox in nature. 38
  • 39. PROCESSING  Mechanical grading can be done with cleaner cum grader, which will remove the undesired immature and chaffy seeds.  The middle screen size should be 9/64’’ round perforated sieves. The size can vary depending on the type of seed.  For fodder sorghum 8/64’’ sieve is used. 39
  • 40. SEED TREATMENT (FOR STORAGE)  The seeds are infested with several storage pests, to protect against these pests the seeds are given protective treatment with bavistin @2g/kg of seed with carbaryl @200mg/kg of seed as slurry treatment.  Bifenthrin @5mg/kg of seed is also recommended for fodder sorghum. 40
  • 41. SEED PACKING & STORAGE  Seeds are packed in gunny bags for short term storage while in HDPE and polylined gunny bag for long term storage.  The treated seed can be stored up to 12 months provided the seeds are not infected with storage pests.  Seed can be stored up to 3 years if the seeds are packed in moisture containers and are stored at low temperature.  The godown should be kept clean as the possibility of secondary infestation with Trifolium(red flour weevil) is much in these crop. 41
  • 42. SOME IMPORTANT HYBRID VARIETIES HYBRID PARENTS CSH1 CK 60 A x IS 84 CSH5 2077A x CS3541 CSH9 MS 296 x CS 3541 COH2 2219A x IS3541(Kovilpatti Tall) COH3 2077A x CO21 COH4 296A x TNS30 CSH 13 R 296 A x RS 29 CSH14 AKMS 14A x AKR 150 CSH16 27 A x C43 CSH15 (R) 104 A x R 585 CSH17 AKMS 14A x RS 673 42
  • 43. SEED STANDARD FOUNDATION SEED CERTIFIED SEED Physical Purity(%) 98 98 Inert matter(%) 2 2 Other crop seed 5/kg 5/kg Weed seed 10/kg 20/kg Other distinguishable variety 10/kg 20/kg Ergot disease by number 0.020% 0.040% Moisture content Moisture previous container 12 12 Moisture vapour proof container 8 8 43
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