Western Blot Customer Review Anti-Phospho-Cofilin (S3) Antibody (STJ90230)
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The document reviews the usage of an anti-phospho-cofilin (S3) antibody for Western blotting, highlighting its effectiveness in detecting phosphorylated cofilin in HEK293T cells. It provides detailed experimental protocols including dilution factors, transfer methods, and visualization timing. The antibody demonstrated a clear band at 18-19 kDa, indicating successful application.
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Western Blot Customer Review Anti-Phospho-Cofilin (S3) Antibody (STJ90230)
- 1. Rating: Specificity: ANTIBODY CUSTOMER REVIEW Findings:“It is a good antibody for western blotting” – External Lab Knowledge Dock Business Centre, University Way, London E16 2RD T: (+44) 0208 223 3081 F: (+44) 0207 681 2580 E: [email protected] w: www.stjohnslabs.com Protocol Gel Electrophoresis 15% SDS-PAGE gel to run samples. Membrane Wash Washed with PBS +0.05% TWEEN 20 for four time Transfer Transfer to PVDF membrane. Secondary Antibody Probing Secondary antibody HRP conjugated anti-rabbit 1:5000 for 1 hour at RT. Blocking 5% Milk Membrane Wash Washed with PBS +0.05% TWEEN 20 for four time Primary Antibody Probing Primary antibody, dilution with 1:20000, incubate overnight. Visualisation ECL visualization was undertaken after 5 sec, 10 sec, 1 min, 5min, 20 min and 1hr. Model Number STJ90230 Antibody Name Anti-Phospho-Cofilin (S3) antibody Application Western Blot Species Human Tissue HEK293T Dilution 1:20000 Secondary Antibody HRP conjugated anti- rabbit Secondary Dilution 1:5000 Fig 1. we used this antibody to detect p-cofilin in HEK293T cells and could detect a band around 18-19 KD.